Methods and compositions for modulating keratinocyte function

ABSTRACT

The disclosure is generally directed to methods and compositions for modulating keratinocyte function, more particularly, to compositions and methods for normalizing keratinocyte proliferation and differentiation, compositions and methods for modulating levels of phosphatidylglycerol (PG) in keratinocytes, and compositions and methods for treating skin conditions by modulating keratinocyte proliferation.

CROSS-REFERENCE TO RELATED APPLICATION

This application is a continuation-in-part of U.S. application Ser. No.11/791,388, which is the National Stage of International Application No.PCT/US2005/042748, filed Nov. 23, 2005, which claims the benefit of U.S.Provisional Application No. 60/635,565, filed Nov. 23, 2004 each ofwhich are entirely incorporated herein by reference.

STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT

Aspects of this disclosure were supported in part by the NationalInstitutes of Health Grant Nos. AR45212 and AR55022. The United StatesGovernment may have certain rights with respect to the claimed subjectmatter.

FIELD OF THE DISCLOSURE

The disclosure is generally directed to methods and compositions formodulating keratinocyte function, more particularly, to compositions andmethods for normalizing keratinocyte proliferation and differentiation.

BACKGROUND

The skin is the largest organ of the body and is composed of theepidermis and dermis. The most important function of the skin is toprovide the essential physical and water permeability barrier. Theepidermis is a continuously regenerating tissue, which differentiates toproduce a mechanical and water permeability barrier, thus makingpossible a terrestrial existence. This barrier is established in theepidermis by a precisely regulated keratinocyte differentiation programthat results in distinct epidermal layers. The structure of theepidermis is maintained by a finely tuned balance between keratinocyteproliferation and differentiation, which results in a multilayerstructure consisting of basal, spinous, granular, and cornified layers.

The innermost basal layer, which is in contact with the basementmembrane, is composed of a single layer of undifferentiatedkeratinocytes with proliferative potential. The spinous layer consistsof non-proliferating keratinocytes in an early differentiation stagewith progressive maturation as the cells move from suprabasal layersoutward. Spinous differentiation is followed by late differentiation inthe granular layer and terminal differentiation in the outermostcornified layer (see FIG. 1). Once committed to differentiation, thecells in the basal layer lose their proliferative potential and movetoward the terminally differentiated cornified layer. Despite intenseinvestigation and data implicating elevated extracellular calciumlevels, 1,25-dihydroxyvitamin D₃ and other molecules, the exactmechanisms by which the keratinocyte differentiation process isinitiated and regulated remain unclear.

The precise regulation of differentiation in the epidermis is crucialfor proper stratification and barrier formation to occur. Epidermalhomeostasis is maintained in part by orchestrating the correctexpression of genes in keratinocytes at each stage of differentiation.Alterations in this differentiation program can result in skindisorders, such as psoriasis, eczema, atopic dermatitis, skin cancers,such as squamous and basal cell carcinoma, and other conditions of theskin characterized by unregulated cell division.

Thus, any upset in the balance of skin cell proliferation anddifferentiation signals can result in various disorders or otherundesirable skin conditions. While an over-stimulation of keratinocyteproliferation may lead to hyperproliferative skin conditions, such asthose mentioned above (i.e. psoriasis and various non-melanoma skincancers), under-stimulation of keratinocyte proliferation may result ina situation of reduced growth, such as that characterized by aging skin(skin cell senescence) or skin that has been damaged. Thus, treatmentsdirected at reducing and/or inhibiting proliferation of keratinocyteswould be useful for treating conditions characterized byhyperproliferation of skin cells. Likewise, treatments for increasingproliferation of keratinocytes would be useful to improve the conditionof aging or damaged skin, where new growth is slowed, and/or toaccelerate wound healing. Particularly beneficial treatments wouldprovide the ability to treat both conditions simultaneously or asneeded; however no such treatments are currently available.

Accordingly, there is a need for new and effective treatments forconditions and/or diseases related to an over- or under-proliferation ofskin cells. There is also a need for ways to modulate keratinocyteproliferation and/or behavior. In particular, there is a need for newmethods and treatments to normalize keratinocyte proliferation.

SUMMARY

Briefly described, the present disclosure provides methods andcompositions for normalizing keratinocyte function and/or proliferation.Aspects of the present disclosure also include modulating keratinocytefunction, and/or modulating levels of phosphatidylglycerol (PG), or afunctional derivative thereof, in keratinocytes. In addition, thepresent disclosure provides methods and compositions for treating skinconditions by modulating keratinocyte proliferation.

Accordingly, embodiments of methods according to the present disclosurefor modulating keratinocyte function include modifying the amount of PG,or a functional derivative thereof, in keratinocytes. Other embodimentsinclude methods for modulating keratinocyte function includingcontacting a keratinocyte with an amount of PG, a functional derivativethereof, a prodrug of the any of the foregoing or a pharmaceuticallyacceptable salt of the any of the foregoing, effective to modulatesignal transduction in the keratinocyte. Embodiments of methods ofmodulating production of phosphatidic acid and PG include contactingkeratinocytes with a non-glycerol based alcohol.

Further, embodiments of the present disclosure for treating a skincondition include administering to a host an amount of PG, a functionalderivative thereof, a prodrug of the any of the foregoing or apharmaceutically acceptable salt of the any of the foregoing, in anamount effective to treat the skin disorder. Other embodiments oftreating a skin condition in a host include increasing the amount of PGin host keratinocytes. Methods of treating a skin condition in a hostalso include administering to the host an amount of PG effective totreat the skin condition, wherein the PG stimulates skin cellproliferation when the skin condition is characterized byunder-proliferation of skin cells, and inhibits skin cell proliferationwhen the skin condition is characterized by over-proliferation of skincells.

Embodiments of methods of normalizing keratinocyte proliferation in ahost include administering to the host an effective amount of PG, afunctional derivative thereof, a prodrug of the any of the foregoing ora pharmaceutically acceptable salt of the any of the foregoing, whereinthe foregoing stimulates keratinocyte proliferation under conditions ofreduced proliferation, and wherein the foregoing inhibits keratinocyteproliferation under conditions of over-proliferation. The presentdisclosure also provides methods of accelerating wound healing in a hostincluding increasing the amount of PG or a functional derivative thereofin host keratinocytes.

The present disclosure also provides compositions for treating variousskin conditions. Embodiments of compositions of the present disclosureinclude an amount of PG, a functional derivative thereof, a prodrug ofthe any of the foregoing or a pharmaceutically acceptable salt of theany of the foregoing, effective to modulate skin cell signaltransduction. Other embodiments of compositions of present disclosureinclude an amount of liposomes of PG, a functional derivative thereof, aprodrug of the any of the foregoing or a pharmaceutically acceptablesalt of the any of the foregoing effective to modulate skin cell signaltransduction.

Other systems, methods, features, and advantages of the presentdisclosure will be or will become apparent to one with skill in the artupon examination of the following drawings and detailed description. Itis intended that all such additional systems, methods, features, andadvantages be included within this description, be within the scope ofthe present disclosure, and be protected by the accompanying claims.

BRIEF DESCRIPTION OF THE DRAWINGS

Many aspects of the disclosure can be better understood with referenceto the following drawings. The components in the drawings are notnecessarily to scale, emphasis instead being placed upon clearlyillustrating the principles of the present disclosure.

FIG. 1 is an illustration of the layers of the skin and the stages ofproliferation and differentiation of keratinocytes.

FIG. 2 illustrates the transphosphatidylation reaction of PLD. In thepresence of water, PLD catalyzes the hydrolysis of the phospholipidphosphatidylcholine to yield phosphatidic acid (PA) and choline.However, in the presence of small amounts of a primary alcohol such asethanol, 1-butanol, or glycerol, PLD catalyzes a transphosphatidylationreaction to produce the corresponding phosphatidylalcohol.

FIG. 3 illustrates PLD signaling pathways, including regulation, signalgeneration, and effector enzymes.

FIG. 4 is a model of the AQP3-PLD2-glycerol-phosphatidylglycerolsignaling module.

FIGS. 5 A and B illustrate that glycerol serves as a substrate forphospholipase D in the transphosphatidylation reaction in vitro.Liposomes were prepared from [³H-dipalmitoyl]phosphatidylcholine,phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol4,5-bisphosphate by sonication. Glycerol at the indicated concentrationsin the absence (A) or presence of 1% ethanol (B) was added to thereaction mix. Reactions were initiated by the addition of Sf9PLD2-overexpressing membranes (1 μg protein), incubated for 30 minutesat 37° C. and terminated by the addition of 0.2% SDS (±5 mM EDTA).Lipids were extracted, separated, and quantified. The figure isrepresentative of at least two additional experiments. There was somevariability in the absolute levels of phosphatidic acid (PA), PG andphosphatidylethanol (PEt) formed, likely due to variations in the extentof formation of multilamellar vesicles during sonication.

FIG. 6 demonstrates that phosphatidylglycerol formation is increased indifferentiating cells exposed to elevated extracellular calciumconcentrations but not 1,25-dihydroxyvitamin D₃. Near-confluentkeratinocytes were incubated with (A) 25 μM-calcium-SFKM containingvehicle (Con; 0.05% ethanol), 250 nM 1,25-dihydroxyvitamin D₃ (D₃), or125 μM calcium (+0.05% ethanol; Ca²⁺) for 24 hours. 2.5-5 μCi/well[³H]glycerol were then added for an additional 30 minutes at 37° C.Reactions were terminated by the addition of 0.2% SDS (+5 mM EDTA) andphospholipids extracted, separated, and quantified. Results areexpressed as -fold over the control value and represent the means±SEM of3 separate experiments; *p<0.001 versus the control. The thin-layerchromatogram shown in Panel B is representative of the three experimentsquantified in Panel A.

FIGS. 7A and B show that elevated extracellular calcium concentrationincreases phosphatidylglycerol production, and to a lesser extentglycerol uptake, in a dose-dependent manner. Near-confluentkeratinocytes were incubated with SFKM containing various concentrationsof calcium for 24 hours. (A) The cells were then incubated for anadditional 30 minutes with 5 μCi/well [³H]glycerol prior to terminationof reactions with 0.2% SDS (±5 mM EDTA) and extraction, separation, andquantification of radiolabeled PG. Values are expressed as -fold overthe control (25 μM-calcium-SFKM) and represent the means±SEM of 5separate experiments; *p<0.05 versus the control value. (B) After a24-hour pretreatment with various calcium concentrations, the cells wereincubated for 5 minutes with 1 μCi/well [³H]glycerol in SFKM containing20 mM HEPES, prior to termination of reactions by extensive washing withice-cold phosphate-buffered saline lacking divalent cations. Values areexpressed as -fold over the control (25 μM-calcium-SFKM) and representthe means±SEM of 5 separate experiments; **p<0.01, *p<0.05 versus thecontrol value.

FIG. 8 is a bar graph showing that phosphatidylglycerol formation isinhibited in differentiating cells exposed to intermediate and highconcentrations of 1,25-dihydroxyvitamin D₃. Near-confluent keratinocyteswere incubated with SFKM containing 0.05% ethanol (Con), 10 nM1,25-dihydroxyvitamin D₃, or 250 nM 1,25-dihydroxyvitamin D₃ (D₃) for 24hours. 2.5-5 μCi/well [³H]glycerol were then added for an additional 30minutes at 37° C. Reactions were terminated by the addition of 0.2% SDS(+5 mM EDTA) and phospholipids extracted, separated, quantified asdescribed in Methods and expressed as -fold over the control value.Results represent the means±SEM of 3 separate experiments; *p<0.01,**p<0.001 versus the control.

FIG. 9 is a bar graph illustrating that the extracellular calciumconcentration-stimulated phosphatidylglycerol formation is inhibited byethanol. Near-confluent keratinocytes were incubated with 25 μM-calciumSFKM (control) or 125 μM-calcium SFKM for 24 hours. The cells were thenincubated for an additional 30 minutes with 0.5-1 μCi/well [¹⁴C]glycerolin the presence and absence of 1% ethanol. Reactions were terminated bythe addition of 0.2% SDS 5 mM EDTA), and radiolabeled PG was extracted,separated by thin-layer chromatography and quantified. Values areexpressed as -fold over the control (without ethanol) and represent themeans±SEM of 4 separate experiments; *p<0.01, **p<0.001 versus thecontrol value, ° p<0.01 versus 125 μM calcium-SFKM alone.

FIG. 10 shows that increased radiolabel was released by bacterialphospholipase D from phosphatidylglycerol isolated from elevatedextracellular calcium-pretreated versus control cells. Near-confluentkeratinocytes were incubated with 25 μM-calcium SFKM (control) or 125μM-calcium SFKM for 24 hours. The cells were then incubated for anadditional 30 minutes with 1 μCi/well [¹⁴C]glycerol, followed byextraction of the lipids into chloroform/methanol and separation of PGby thin-layer chromatography. After solubilization, PG isolated fromcontrol (Con) or 125 μM calcium-treated (Ca²⁺) cells was incubated with(PLD) or without (H₂O) bacterial PLD, and the radioactivity remaining inPG (light striped bars) and phosphatidic acid (dark striped bars) wasquantified after thin-layer chromatographic separation. Values representthe means±SEM from three experiments; *p<0.001 versus the correspondinguntreated control value, °p<0.001 versus the corresponding untreatedcalcium-treated value.

FIG. 11 is a bar graph showing that Phorbol 12-myristate 13-acetate(PMA) does not induce phosphatidylglycerol formation despite activatingPLD. Near-confluent keratinocytes were incubated without radiolabel (forphosphatidylglycerol production) or pre-labeled with 2.5 μCi/mL[³H]oleate (for phosphatidylethanol formation) for 20-24 hours. Thecells were then stimulated for 30 minutes with vehicle (0.05-0.1% DMSO;Con) or 100 nM PMA in the presence of [³H]glycerol (forphosphatidylglycerol production), or in the presence of 0.5% ethanol(for phosphatidylethanol formation). Reactions were terminated by theaddition of 0.2% SDS (±5 mM EDTA) and radiolabeled phosphatidylglycerol(PG), or phosphatidylethanol (PEt) was extracted, separated bythin-layer chromatography and quantified. Values are expressed as -foldover control and represent the means±SEM of three separate experimentsperformed in duplicate or triplicate; *p<0.02 versus the appropriatecontrol by an unpaired Student's t-test.

FIG. 12 illustrates that pretreatment, but not simultaneous incubation,with PMA inhibits [³H]glycerol uptake. Glycerol uptake was measured incells pretreated or treated simultaneously with and without PMA. For the“no pretreatment” samples, cells were incubated for 5 minutes in SFKMcontaining 20 mM HEPES, 1 μCi/mL [³H]glycerol and 0.1% DMSO (control) or100 nM PMA. For the “30-minute pretreatment with PMA” samples, confluentkeratinocytes were pre-incubated for 30 minutes in SFKM containing 0.1%DMSO (control) or 100 nM PMA. Cells were then incubated for 5 minutes inSFKM containing 20 mM HEPES and 1 μCi/mL [³H]glycerol. For both sets ofsamples, radiolabeled glycerol uptake was measured. Values represent themeans of 3 (no pretreatment) or 5 (30-minute pretreatment) separateexperiments performed in duplicate or triplicate; *p<0.001 versus thecontrol value of 100% (dotted line).

FIGS. 13A and B illustrate that an extracellular medium of pH 4 inhibitsradiolabeled glycerol uptake (A) and PG synthesis (B). Keratinocyteswere pretreated for 24 hours with control (25 μM Ca²⁺) medium (Con) or125 μM Ca²⁺ (Ca²⁺)-containing medium. Some cells were then incubated for5 (panel A) minutes with medium of pH 4 prior to (A) measurement of [³H]glycerol uptake for 5 minutes, or (B) [¹⁴C]PG synthesis for 10 minutes,at pH 4 or 7 (7.4) as indicated. Results represent the means±SEM of (A)four or (B) three experiments performed in duplicate; *p<0.05, **p<0.001versus the control value (glycerol uptake or PG synthesis in controlcells measured at pH 7); †p<0.01, ††p<0.001 versus the Ca²⁺ valuemeasured at pH 7 (7.4). Note that the effects of low pH on [³H]glyceroluptake (panel A) and [¹⁴C]PG synthesis (panel B) were essentiallyreversible (compare pH 7 to pH 4/7).

FIGS. 14A-C are bar graphs demonstrating that AQP3 overexpressiondecreases keratin 5 promoter activity, increases keratin 10 promoteractivity and enhances the effect of elevated [Ca²⁺], on involucrinpromoter activity. Primary keratinocytes were co-transfected with pcDNA3vector alone (control) or the vector possessing AQP3 and (A) the keratin5 promoter/reporter gene construct or (B) the involucrinpromoter/reporter gene constructs (and pRL-SV40 for normalizationpurposes) using TransIT-Keratinocyte as described by the manufacturer.After 24 hours, cells were re-fed with medium containing 25 μM (control)or 1 HiM-Ca²⁺ for an additional 24 hours. Luciferase activity was thenmeasured using a Dual Luciferase kit as directed by the manufacturer.Activity is expressed relative to the pcDNA3-transfected control cellsand represents the mean±SEM of three experiments performed intriplicate; *p<0.01, **p<0.001 versus the control (untreated pcDNA3vector) value, †p<0.01, ††p<0.001 versus the AQP3-transfected valueunder control conditions, and §p<0.001 versus the Ca²⁺-treated pcDNA3vector control value.

FIGS. 15A-B illustrate that glycerol, but not xylitol or sorbitol,inhibits DNA synthesis and enhances the inhibitory effect of an elevatedextracellular Ca²⁺ concentration. (A) Near-confluent keratinocytes wereincubated for 24 hours with 0.02 or 0.1% glycerol and DNA synthesismeasured as the incorporation of [³H] thymidine incorporation into DNA.(B) Near-confluent keratinocytes were incubated for 24 hours with theindicated concentrations of glycerol (G, squares) or equivalentconcentrations of xylitol (X, circles) in SFKM containing 25 μM(control; open symbols) or 125 μM Ca²⁺ (Ca²⁺; closed symbols). (C)Near-confluent keratinocytes were incubated for 24 hours with theindicated concentrations of glycerol (G) or equivalent concentrations ofsorbitol (S, triangles) in SFKM containing 25 μM (control; open symbols)or 125 μM Ca²⁺ (Ca²⁺; closed symbols) for 24 hours. [³H]Thymidineincorporation into DNA was then determined. Values represent themeans±SEM of 4 to 5 separate experiments performed in duplicate;*p<0.05, **p<0.01 versus the control value, †p<0.05 versus the value inthe presence of Ca²⁺ alone. (D) Primary keratinocytes wereco-transfected with pcDNA3 vector alone (control) or the same vectorpossessing AQP3 and the keratin 5 promoter/reporter construct (andpRL-SV40 for normalization purposes) using TransIT-Keratinocyte asdescribed by the manufacturer. After 24 hours cells were refed withmedium containing no addition (control) or 0.2% glycerol for anadditional 24 hours. Luciferase activity was then measured using a DualLuciferase kit as directed by the manufacturer. Activity is expressedrelative to the pcDNA3-transfected control cells and represents themean±SEM of 5 experiments performed in triplicate; *p<0.05, **p<0.01,***p<0.001 versus the control (untreated pcDNA3 vector) value; †p<0.05versus the AQP3-transfected value under control conditions. (E)Near-confluent keratinocytes were incubated for 24 hours with theindicated concentrations of PG, prepared via bath sonication of PG inSFKM, in 25 μM Ca²⁺-containing medium (control) or medium containing 125μM Ca²⁺. Cells were harvested and subject to western analysis using ananti-involucrin antibody (Covance) and the LiCor Odyssey system. Theblots were quantified using the Kodak molecular imaging software. Valuesrepresent the mean±SEM of 3-4 separate experiments performed induplicate; *p<0.05, **p<0.01 versus the control value.

FIG. 16 demonstrates that 1-,2-propylene glycol (1,2-propanediol)inhibits DNA synthesis and enhances the inhibitory effect of an elevatedextracellular Ca²⁺ concentration. (A) Near-confluent keratinocytes wereincubated for 24 hours with the indicated concentrations of glycerol (G,squares) or equivalent concentrations of 1,2-propylene glycol(1,2-propanediol, triangles) in SFKM containing 25 μM (control; opensymbols) or 125 μM Ca²⁺ (Ca²⁺; closed symbols). [³H]Thymidineincorporation into DNA was then determined as in [3]. Values representthe means±SEM of 3 to 5 separate experiments performed in duplicate;*p<0.05, **p<0.01 versus the control value, †p<0.05 versus the value inthe presence of Ca²⁺ alone. (B) The structures of glycerol and1,2-propylene glycol demonstrate the similarity of their configuration.

FIG. 17 illustrates that PG liposomes, but not PP liposomes, inhibit DNAsynthesis in proliferating keratinocytes and PG liposomesdose-dependently stimulate transglutaminase activity. (A) Near-confluentkeratinocytes were treated for 24 hours with the indicatedconcentrations of phosphatidylglycerol (PG), prepared via bathsonication of PG in serum-free keratinocyte medium. [³H]Thymidineincorporation into DNA was then determined. [³H]Thymidine incorporationinto DNA in the control was 85,550±5,730 cpm/well. Values represent themeans±SEM of 7-9 separate experiments performed in duplicate; *p<0.01,**p<0.001 versus the control value. (B) Near-confluent keratinocyteswere treated for 24 hours with the indicated concentrations ofphosphatidylglycerol (PG), prepared via bath sonication of PG inserum-free keratinocyte medium. Transglutaminase activity was thendetermined. Values represent the means±SEM of separate experimentsperformed in duplicate; the increasing doses exhibited a significantstimulatory trend; *p<0.05. (C) Near-confluent keratinocytes weretreated for 24 hours with the indicated concentrations ofphosphatidylglycerol (PG) or phosphatidylpropanol (PP), prepared viabath sonication of PG or PP in serum-free keratinocyte medium.[³H]Thymidine incorporation into DNA was then determined. Valuesrepresent the means±SEM of 5-6 separate experiments performed induplicate; *p<0.05, **p<0.001 versus the control value.

FIG. 18 shows that PG liposomes increase DNA synthesis ingrowth-inhibited keratinocytes. Confluent keratinocytes were treated for24 hours with the indicated concentrations of phosphatidylglycerol (PG),prepared via bath sonication of PG in serum-free keratinocyte medium.[³H]Thymidine incorporation into DNA was then determined as above.[³H]Thymidine incorporation into DNA under control conditions was12,880±1,040 cpm/well. Values represent the means±SEM of 3 separateexperiments performed in duplicate; *p<0.01, **p<0.001 versus thecontrol value.

FIG. 19 is a bar graph showing the effect of glycerol andphosphatidylglycerol on the rate of wound healing.

FIG. 20 illustrates that PG liposomes containing selected fatty acidmolecules at the R₁ and R₂ positions impact the ability of PG tonormalize keratinocyte proliferation. (A) Rapidly proliferatingkeratinocytes were treated for 24 hours with the indicatedconcentrations and species of phosphatidylglycerol (PG), prepared viabath sonication of PG in serum-free keratinocyte medium. [³H]Thymidineincorporation into DNA was then determined. Values represent the means(±SEM for egg-derived PG) of at least four separate experiments. PGspecies tested were DHPG (dihexanoyl-PG, 6:0-6:0), DPPG (dipalmitoyl-PG,16:0-16:0), DOPG (dioleoyl-PG, 18:1-18:1), DSPG (distearoyl-PG,18:0-18:0), POPG (palmitoyl-oleoyl-PG, 16:0-18:1), egg-derived PG, PLPG(palmitoyl-linoleoyl-PG, 16:0-18:2), soy-derived PG, PAPG(palmitoyl-arachidonoyl-PG, 16:0-20:4) and DLPG (dilinoleoyl-PG,18:2-18:2). (B) Slowly proliferating keratinocytes were treated for 24hours with the indicated concentrations and species ofphosphatidylglycerol (PG), prepared via bath sonication of PG inserum-free keratinocyte medium. [³H]Thymidine incorporation into DNA wasthen determined. Values represent the means (±SEM for egg-derived PG) ofat least four separate experiments. PG species tested were DHPG(dihexanoyl-PG, 6:0-6:0), DPPG (dipalmitoyl-PG, 16:0-16:0), DOPG(dioleoyl-PG, 18:1-18:1), POPG (palmitoyl-oleoyl-PG, 16:0-18:1),egg-derived PG and PAPG (palmitoyl-arachidonoyl-PG, 16:0-20:4).

FIG. 21 shows the headgroup of the phosphatidylalcohol impacts theability to normalize keratinocyte proliferation. (A) Shows the structureof glycerol and 1-propanol. (B) Near-confluent keratinocytes weretreated for 24 hours with the indicated concentrations and species ofphosphatidylglycerol (PG) or phosphatidylpropanol (PP), prepared viabath sonication of PG or PP in serum-free keratinocyte medium.[³H]Thymidine incorporation into DNA was then determined. Species testedwere dipalmitoyl-PG (DPPG, 16:0-16:0), dioleoyl-PG (DOPG, 18:1-18:1),dilinoleoyl-PG (DLPG, 18:2-18:2), dipalmitoyl-PP (DPPP, 16:0-16:0),dioleoyl-PP (DOPP, 18:1-18:1) and dilinoleoyl-PP (DLPP, 18:2-18:2).Values are expressed as percent of control (with control being noaddition of any PG or PP species). Values represent the means±SEM of atleast three separate experiments. The asterisk indicates a statisticallysignificant difference from control (p<0.05).

DETAILED DESCRIPTION

Embodiments of the present disclosure will employ, unless otherwiseindicated, techniques of synthetic organic chemistry, biochemistry,molecular biology, and the like, which are within the skill of the art.Such techniques are explained fully in the literature. The followingexamples are put forth so as to provide those of ordinary skill in theart with a complete disclosure and description of how to perform themethods and use the compositions and compounds disclosed and claimedherein. Efforts have been made to ensure accuracy with respect tonumbers (e.g., amounts, temperature, etc.), but some errors anddeviations should be accounted for. Unless indicated otherwise, partsare parts by weight, temperature is in ° C., and pressure is at or nearatmospheric. Standard temperature and pressure are defined as 20° C. and1 atmosphere.

Before the embodiments of the present disclosure are described indetail, it is to be understood that, unless otherwise indicated, thepresent disclosure is not limited to particular materials, reagents,reaction materials, manufacturing processes, or the like, as such canvary. It is also to be understood that the terminology used herein isfor purposes of describing particular embodiments only, and is notintended to be limiting. It is also possible in the present disclosurethat steps can be executed in different sequence where this is logicallypossible.

It must be noted that, as used in the specification and the appendedclaims, the singular forms “a,” “an,” and “the” include plural referentsunless the context clearly dictates otherwise. Thus, for example,reference to “a support” includes a plurality of supports. In thisspecification and in the claims that follow, reference will be made to anumber of terms that shall be defined to have the following meaningsunless a contrary intention is apparent.

Definitions

As used herein, the term “host” or “organism” includes both humans,mammals (e.g., cats, dogs, horses, etc.), and other living species thatare in need of treatment for conditions/diseases of the skin. A livingorganism can be as simple as, for example, a single eukaryotic cell oras complex as a mammal. Further, a “composition” can include one or morechemical compounds, as described below.

As used herein, the term “derivative” refers to a modification to thedisclosed compounds including, but not limited to, hydrolysis,reduction, or oxidation products, of the disclosed compounds.Hydrolysis, reduction, and oxidation reactions are known in the art.

As used herein, the term “functional derivative” refers to a derivativeof the disclosed compounds that retains the function of the disclosedcompound, at least in part. For instance, in the case of PG, afunctional derivative of PG in the context of the present disclosureincludes a derivative of PG which has the effect of modulating skin cellsignal transduction and/or proliferation. A non-limiting example of afunctional derivative of PG in the present disclosure is thephosphatidylalcohol formed upon transphosphatidylation using propyleneglycol or 1-propanol, which has the same chemical structure of PG minusone or two hydroxyl groups, respectively, and which retains the activityof PG, at least in part.

As used herein, the term “therapeutically effective amount” refers tothat amount of the compound being administered which will relieve tosome extent one or more of the symptoms caused directly or indirectly byan over- or under-proliferation of keratinocytes. In reference toconditions/diseases caused directly or indirectly by an over- orunder-proliferation of keratinocytes, a therapeutically effective amountrefers to that amount which has the effect of preventing thecondition/disease from occurring in an animal that may be predisposed tothe disease but does not yet experience or exhibit symptoms of thecondition/disease (prophylactic treatment), alleviation of symptoms ofthe condition/disease, diminishment of extent of the condition/disease,stabilization (i.e., not worsening) of the condition/disease, preventingthe spread of condition/disease, delaying or slowing of thecondition/disease progression, amelioration or palliation of thecondition/disease state, and combinations thereof.

As used herein, the term “pharmaceutically acceptable salt” refers tothose salts that retain the biological effectiveness and properties ofthe free bases and which are obtained by reaction with inorganic ororganic acids such as, but not limited to, hydrochloric acid,hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid,methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid,salicylic acid, malic acid, maleic acid, succinic acid, tartaric acid,citric acid, and the like.

As used herein, the term “pharmaceutical composition” refers to amixture of one or more of the compounds described herein, orpharmaceutically acceptable salts thereof, with other chemicalcomponents, such as physiologically acceptable carriers and excipients.One purpose of a pharmaceutical composition is to facilitateadministration of a compound to an organism.

As used herein, a “pharmaceutically acceptable carrier” refers to acarrier or diluent that does not cause significant irritation to anorganism and does not abrogate the biological activity and properties ofthe administered compound.

As used herein, the term “excipient” refers to an inert substance addedto a pharmaceutical composition to further facilitate administration ofa compound. Examples of excipients include, but are not limited to,calcium carbonate, calcium phosphate, various sugars and types ofstarch, cellulose derivatives, gelatin, vegetable oils, and polyethyleneglycols.

As used herein, “treat,” “treating,” and/or “treatment” are an approachfor obtaining beneficial or desired clinical results. For purposes ofembodiments of this disclosure, beneficial or desired clinical resultsinclude, but are not limited to, preventing the condition/disease fromoccurring in an animal that may be predisposed to the condition/diseasebut does not yet experience or exhibit symptoms of the disease(prophylactic treatment), alleviation of symptoms of thecondition/disease, diminishment of extent of the condition/disease,stabilization (i.e., not worsening) of the condition/disease, preventingspread of the condition/disease, delaying or slowing of thecondition/disease progression, amelioration or palliation of thecondition/disease state, and combinations thereof, hi addition, “treat”,“treating”, and “treatment” can also mean prolonging survival ascompared to expected survival if not receiving treatment.

As used herein, the term “prodrug” refers to an agent that is convertedinto a biologically active form in vivo. Prodrugs are often usefulbecause, in some situations, they may be easier to administer than theparent compound. They may, for instance, be bioavailable by oraladministration whereas the parent compound is not. The prodrug may alsohave improved solubility in pharmaceutical compositions over the parentdrug. A prodrug may be converted into the parent drug by variousmechanisms, including enzymatic processes and metabolic hydrolysis.Harper, N.J. (1962). Drug Latentiation in Jucker, ed. Progress in DrugResearch, 4:221-294; Morozowich et al. (1977). Application of PhysicalOrganic Principles to Prodrug Design in E. B. Roche ed. Design ofBiopharmaceutical Properties through Prodrugs and Analogs, APhA; Acad.Pharm. ScL; E. B. Roche, ed. (1977). Bioreversihle Carriers in DrugDesign, Theory and Application, APhA; H. Bundgaard, ed. (1985) Design ofProdrugs, Elsevier; Wang et al. (1999) Prodrug approaches to theimproved delivery of peptide drug, Curr. Pharm. Design. 5(4):265-287;Pauletti et al. (1997). Improvement in peptide bioavailability:Peptidomimetics and Prodrug Strategies, Adv. Drug. Delivery Rev.27:235-256; Mizen et al. (1998). The Use of Esters as Prodrugs for OralDelivery of β-Lactam antibiotics, Pharm. Biotech. 1 1,:345-365;Gaignault et al. (1996). Designing Prodrugs and Bioprecursors I. CarrierProdrugs, Pract. Med. Chem. 671-696; M. Asgharnejad (2000). ImprovingOral Drug Transport Via Prodrugs, in G. L. Amidon, P. I. Lee and E. M.Topp, Eds., Transport Processes in Pharmaceutical Systems, MarcellDekker, p. 185-218; Balant et al. (1990). Prodrugs for the improvementof drug absorption via different routes of administration, Eur. J.DrugMetab. Pharmacokinet., 15(2): 143-53; Balimane and Sinko (1999).Involvement of multiple transporters in the oral absorption ofnucleoside analogues, Adv. Drug Delivery Rev., 39(1-3):183-209; Browne(1997). Fosphenyloin (Cerebyx), Clin. Neuropharmacol. 20(1): 1-12;Bundgaard (1979). Bioreversible derivatization of drugs—principle andapplicability to improve the therapeutic effects of drugs, Arch. Pharm.Chemi. 86(1): 1-39; H. Bundgaard, ed. (1985). Design of Prodrugs, NewYork: Elsevier; Fleisher et al. (1996). Improved oral drug delivery:solubility limitations overcome by the use of prodrugs, Adv. DrugDelivery Rev. 19(2): 115-130; Fleisher et al. (1985). Design of prodrugsfor improved gastrointestinal absorption by intestinal enzyme targeting,Methods Enzymol. 112: 360-81; Farquhar D, et al. (1983). BiologicallyReversible Phosphate-Protective Groups, J. Pharm. Sd., 72(3): 324-325;Han, H. K. et al. (2000). Targeted prodrug design to optimize drugdelivery, AAPS PharmSci., 2(1): E6; Sadzuka Y. (2000). Effective prodrugliposome and conversion to active metabolite, Curr. DrugMetab.,1(1):31-48; D. M. Lambert (2000). Rationale and applications of lipidsas prodrug carriers, Eur. J. Pharm. ScL, 11 Suppl 2:S15-27; Wang, W. etal. (1999). Prodrug approaches to the improved delivery of peptidedrugs. Curr. Pharm. Des., 5(4):265-87.

As used herein, the term “topically active agents” refers tocompositions of the present disclosure that elicit pharmacologicalresponses at the site of application (contact) to a host.

As used herein, the term “topically” refers to application of thecompositions of the present disclosure to the surface of the skin andmucosal cells and tissues.

As used herein, the term “inhibit,” “suppress,” “decrease,” “interfere,”and/or “reduce” (and like terms) generally refers to the act ofreducing, either directly or indirectly, a function, activity, orbehavior relative to the natural, expected, or average or relative tocurrent conditions. For instance, something that inhibits, suppresses,decreases or reduces or interferes with keratinocyte proliferation mightstop or slow the growth of new keratinocytes.

As used herein, the term “increase,” “enhance,” “stimulate,” and/or“induce” (and like terms) generally refers to the act of improving orincreasing, either directly or indirectly, a function or behaviorrelative to the natural, expected, or average or relative to currentconditions. For instance, something that increases, stimulates, inducesor enhances keratinocyte proliferation might induce proliferation ofkeratinocytes that have slowed or stopped proliferating or acceleratethe rate of proliferation over the normal rate.

As used herein, the term “modulate,” “modify,” and/or “modulator”generally refers to the act of directly or indirectlypromoting/stimulating or interfering with/inhibiting a specific functionor behavior. For instance, a modulator of keratinocyte function mightstimulate or increase keratinocyte proliferation or differentiation, ora modulator of keratinocyte function might inhibit or decreasekeratinocyte proliferation or differentiation. In some instances amodulator may increase and/or decrease a certain activity or functionrelative to its natural state or relative to the average level ofactivity that would generally be expected or relative to a current levelof activity.

As used herein, the term “normalize” refers to the act of establishingand/or maintaining a relative balance or equilibrium between two or moreactivities, functions or conditions. For instance to normalizekeratinocyte proliferation generally refers to maintaining a relativebalance between keratinocyte proliferation and differentiation undervarious conditions. Under conditions of over-proliferation, to normalizemight mean to slow or inhibit proliferation, while under conditions ofslowed growth, to normalize might mean to induce or increaseproliferation.

As used herein, the term “expression” refers to the process undergone bya structural gene to produce a polypeptide. It is a combination oftranscription and translation. Thus, to induce or increase expression ofPLD2 or AQP3 refers to increasing or inducing the production of the PLD2or AQP3 polypeptide, which may be done by a variety of approaches, suchas increasing the number of genes encoding for the polypeptide,increasing the transcription of the gene (such as by placing the geneunder the control of a constitutive promoter), or increasing thetranslation of the gene, or a combination of these and/or otherapproaches.

As used herein, the terms “including”, “such as”, “for example” and thelike are intended to refer to exemplary embodiments and not to limit thescope of the present disclosure.

General Discussion

Phospholipase D

Phospholipase D (PLD) is a lipolytic enzyme that has been implicated inmultiple cellular processes including growth, differentiation, vesicletrafficking and cytoskeletal rearrangement. PLDs catalyze the hydrolysisof phosphatidylcholine to generate phosphatidic acid (PA) and choline.PA and its metabolites, diacylglycerol and lysophosphatidic acid, areinvolved in multiple physiological events. In the presence of primaryalcohols, PLD can also catalyze the transphosphatidylation reaction togenerate phosphatidylalcohols. Pursuant to this mechanism, PLD canmetabolize phosphatidylcholine in the presence of a physiologicalprimary alcohol glycerol to yield phosphatidylglycerol (PG); otherprimary alcohols may also be used in this reaction, such as propyleneglycol or 1-propanol, to yield similar products. The reactions of PLDare illustrated in FIG. 2.

Two isoforms of mammalian PLD, PLD1 and PLD2, have been identified. PLD1has a low basal activity and is activated by small G proteins (Arf, Rho,and Rac) and protein kinase C, whereas PLD2 appears to be constitutivelyactive, as demonstrated by transfection into insect cells monitored invitro. Both PLDs use phosphatidylinositol 4,5-bisphosphate (PIP₂) as acofactor and have been shown to be expressed in keratinocytes.1,25-Dihydroxyvitamin D₃, a keratinocyte differentiating agent, inducesPLD1, but not PLD2 expression. FIG. 3 illustrates various signalingpathways of PLD. In HaCaT cells, PLD2 has been located in caveolin-richmembrane microdomains.

The location of PLD2 and its ability to produce phosphatidylglycerol(PG) implicates PLD2 in the modulation of keratinocyte behavior,specifically with respect to signal transduction for regulatingkeratinocyte proliferation and differentiation, as will be discussed ingreater detail below.

Aquaporin 3

Aquaporins (AQP) are a family of small transmembrane water and/orglycerol channels. Currently, eleven mammalian aquaporins (AQP0-10) havebeen identified and characterized. According to their structural andfunctional properties, aquaporins can be divided into two subgroups:“aquaporins”, which transport only water, and “aquaglyceroporins”, whichcan transport both water and glycerol. AQP3, which belongs to theaquaglyceroporin subgroup, is a relatively weak transporter of water butan efficient transporter of glycerol. AQP3 is expressed in kidneycollecting cells, red cells, dendritic cells and epithelial cells from avariety of tissues including the urinary, digestive, and respiratorytracts and the epidermis. In epidermal, tracheal and nasopharyngealepithelium, AQP3 is present in basal cells of the epidermis.

AQP3-deficient mice display selectively reduced glycerol content, aswell as decreased water holding capacity, in the epidermis, impairedskin elasticity, delayed barrier recovery after stratum corneum removaland delayed wound healing, suggesting a role of AQP3 in regulatingkeratinocyte proliferation and differentiation. This phenotype can becorrected by topical or oral application of glycerol but not otherosmotically active molecules, suggesting that the effect is not simply afunction of glycerol's hydrophilic properties. AQP3's ability totransport glycerol, which can be used to produce PG as discussed above,and its location, discussed below, indicate a role for AQP3 in themodulation of PG production and keratinocyte function, which will bediscussed in greater detail below.

PLD2/AQP3/Glycerol/PG Signaling Module

The inventors of the present disclosure have previously shown that inkeratinocytes AQP3 and PLD2 associate in caveolin-rich membranemicrodomains and that the presence of the AQP3 glycerol channel isimportant for normal epidermal function (Zheng, X. and Bollag, W. B.(2003) J. Invest. Dermatol, 121, 1487-1495, which is hereby incorporatedby reference). Caveolae are a subset of lipid raft microdomains, whichare characterized electron microscopically as flask-shaped invaginationsof 50-100 nm diameter in the plasma membrane. Caveolin 1 is the firststructural protein component identified in caveolae and has beenfunctionally implicated in a wide variety of signal transductionprocesses (Smart et al., 1999). In addition, caveolin 1 has recentlybeen shown to associate with lamellar bodies in keratinocytes (Sando etal., 2003).

The colocation of AQP3 with PLD2 in caveolin-rich membrane microdomainssuggests that AQP3 transports glycerol to PLD2 for use in thetransphosphatidylation reaction to produce PG and that PG, in turn, actsas a lipid second messenger to modulate keratinocyte function, which isfurther demonstrated by Examples 1 and 2, below. Indeed, the Examplesherein demonstrate the existence of a novel signaling module comprisedof AQP3, PLD2, glycerol and PG.

Example 2 also demonstrates that direct provision of PG liposomesinhibited DNA synthesis in a dose-dependent fashion in rapidly dividingkeratinocytes, although in growth-inhibited cells, PG liposomesdose-dependently enhanced [³H] thymidine incorporation into DNA. A trendfor stimulation of transglutaminase activity by PG liposomes was alsoobserved. These data support that a signaling module consisting of AQP3,PLD2, glycerol and PG is involved in promoting growth inhibition and/orearly differentiation of proliferating keratinocytes or promoting growthstimulation in growth inhibited keratinocytes, thereby providing amechanism for modulating keratinocyte behavior and/or proliferation andmethods for treating various skin conditions characterized by anincrease or decrease in keratinocyte proliferation.

Methods of Modulating Keratinocyte Proliferation and Treating SkinConditions

Embodiments of the present disclosure include methods of modulatingkeratinocyte function, particularly proliferation, by modulating theamounts and/or activities of the various components of thePLD2/AQP3/glycerol/PG signaling module. In certain embodiments of thepresent disclosure, keratinocyte proliferation is normalized bymodulating the amount of PG, or a functional derivative thereof,produced by or in contact with keratinocytes. In embodiments of thepresent disclosure, modulating the amount of PG, or a functionalderivative thereof, in contact with or produced by, keratinocytesnormalizes keratinocyte proliferation by stimulating skin cellproliferation in conditions of slowed growth or under-proliferation ofskin cells and inhibiting or decreasing skin cell proliferation underconditions of increased growth or hyperproliferation.

Some embodiments of modulating the amount of PG in contact withkeratinocytes include increasing the amount of PG, a functionalderivative thereof, a prodrug or a pharmaceutically acceptable salt ofany of the foregoing, in contact with keratinocytes. Example functionalderivatives of PG include, but are not limited to, thetransphosphatidylation reaction product of other primary alcohols suchas propylene glycol and 1-propanol, which has the same structure as PG,minus one and two hydroxy groups, respectively. Included within themeaning of PG or a functional derivative of PG are those species thatcontain selected fatty acid molecules at the R₁ and R₂ positions. In oneembodiment, the fatty acid molecules are saturated, monounsaturated(containing one unsaturated bond) or polyunsaturated (containing two ormore unsaturated bonds). The nature of the fatty acid molecules at thepositions may be the same or may be different. In one embodiment, thefatty acid molecules contain from 4-28 carbon atoms and from 0-6unsaturated bonds. Exemplary fatty acid molecules, include, but are notlimited to, butyric (4:0), valeric (5:0), caproic (6:0), caprylic (8:0),capric (10:0), lauric (12:0), myristic (14:0), myristoleic (14:1,cis-9), palmitic (16:0), palmitoleic (16:1, 9-cis), stearic (18:0),oleic (18:1, 11-cis), vaccenic (18:1, 11-trans), linoleic (18:2, 9-cis,12-cis), γ-linolenic (18:3, 6-cis, 9-cis, 12-cis), α-linolenic (18:3,9-cis, 12-cis, 15-cis), arachidic (20:0), arachidonic (20:4, 5-cis,8-cis, 11-cis, 14-cis), eicosapentaenoic (20:5, 5-cis, 8-cis, 11-cis,14-cis, 17-cis), behenic (22:0), erucic (22:1, 13-cis), docosahexaenoic(22:6, 4-cis, 7-cis, 10-cis, 13-cis, 16-cis, 19-cis), lignoceric (24:0)and cerotic (26:0). In a particular embodiment, the PG molecule orfunctional derivative thereof contains at least one fatty acid with atleast one unsaturated bond, such as but not limited to oleic orlinoleic.

Embodiments of increasing the amount of PG in contact with keratinocytesto modulate keratinocyte behavior include, contacting keratinocytes withan amount of PG, a functional derivative thereof or a prodrug or apharmaceutically acceptable salt of any of the foregoing effective tomodulate keratinocyte proliferation, keratinocyte skin cell signaltransduction, and/or keratinocyte nucleic acid synthesis. The examplesbelow demonstrate that the PG acts to modulate signal transduction inthe keratinocyte, which can increase or decrease nucleic acid synthesisin the keratinocyte, depending on various conditions.

A surprising and beneficial aspect of the present disclosure is that PGexhibits biphasic action in keratinocytes, inducing signals forproliferation under conditions of slowed growth, such as aging (i.e.cell senescence) or damage to skin cells, such as from exposure tounfavorable conditions (e.g. smoke, sun, wind, and extreme temperatures)or physical injury (such as wounds, burns, scrapes, scars, ulcers,etc.), and inducing signals to inhibit or slow proliferation underconditions of increased or hyper-proliferative growth, such as indisorders including, but not limited to, psoriasis, eczema, actinickeratosis, atopic dermatitis, basal cell carcinoma, and othernon-melanoma skin cancers. Thus, rather than treating conditions ofover- or undergrowth separately, the conditions can be addressedsimultaneously by modulating PG levels and/or production, and orotherwise modulating the PLD2/AQP3/glycerol/PG signaling module.

Methods of the present disclosure are not limited to modulating PGlevels by the administration of PG or primary alcohol, such as but notlimited to, glycerol to keratinocytes or a host, but also includemethods of modulating the amount of PG produced by keratinocytes.Embodiments of modulating the amount of PG produced by keratinocytesinclude modulating the activity of PLD, such as but not limited to,phospholipase D2 (PLD2) and/or AQP, such as but not limited to,aquaporin-3 (AQP3), for example by up-regulating or down-regulating theactivity of such polypeptides and/or increasing or decreasing theexpression of such polypeptides in keratinocytes. Embodiments forincreasing the expression of PLD and/or AQP, such as but not limited to,PLD2 or AQP3, include increasing or inducing the production of the suchpolypeptide, which may be done by a variety of approaches known to thoseof skill in the art, non-limiting examples of which are disclosed belowin the Examples. In general, approaches for increasing expression ofpolypeptides such as PLD2 or AQP3 include methods such as increasing thenumber of genes encoding for the polypeptide (such as by transfection ofhost cells with additional copies of the gene, by various methods knownto those of skill in the art of gene therapy), increasing thetranscription of the gene (such as by placing the gene under the controlof a constitutive promoter), or increasing the translation of the gene,or a combination of these and/or other approaches.

Embodiments of the present disclosure also provide methods andcompositions for treating skin conditions/disorders in a hostcharacterized by over- or under-proliferation of keratinocytes bynormalizing and/or modulating keratinocyte proliferation and/orfunction. Skin conditions treatable by methods and compositions of thepresent disclosure include, but are not limited to hyper-proliferativedisorders such as psoriasis, eczema, actinic keratosis, atopicdermatitis, basal cell carcinoma, non-melanoma skin cancer, andunregulated cell division; conditions of slowed growth such as aging,scarring, skin cell senescence, and skin cell damage due to exposure(such as to sun, smoke, wind, extreme temperatures, etc.); and physicalwounds (such as lacerations, ulcers such as diabetic and age-relatedulcers, burns, scrapes, and the like).

Methods of treating the above conditions include, among others, themethods of modulating/normalizing, keratinocyte proliferation and/orfunction described above. In particular, embodiments of methods fortreating the above conditions include administering an amount of PG, afunctional derivative thereof, or a prodrug or a pharmaceuticallyacceptable salt of any of the foregoing effective to modulatekeratinocyte proliferation, keratinocyte skin cell signal transduction,and/or keratinocyte nucleic acid synthesis. Methods of the presentdisclosure for modulating keratinocyte behavior and/or treating skinconditions may also include, in combination with the administration ofPG, contacting keratinocytes with glycerol or a functional derivativethereof, as described below, to stimulate the cellular production of PGor functional derivatives of PG. Methods of the present disclosure alsoinclude contacting keratinocytes with a non-glycerol based alcohol tomodulate the production of phosphatidic acid, PA, as well as PG asdiscussed in greater detail below. Embodiments of the present disclosurealso include methods of treating the above conditions and modulatingkeratinocyte function and proliferation by administering apharmaceutical composition of the present disclosure to a host in needthereof. Pharmaceutical compositions according to the present disclosureare described in greater detail below.

Pharmaceutical Compositions

Embodiments of pharmaceutical compositions and dosage forms of thepresent disclosure include PG, a pharmaceutically acceptable salt of PGor a functional derivative thereof, or a pharmaceutically acceptablepolymorph, solvate, hydrate, dehydrate, co-crystal, anhydrous, oramorphous form thereof. Embodiments of the pharmaceutical compositionsof the present disclosure may also include glycerol or a functionalderivative thereof. Since glycerol acts as a substrate of PLD2 for theproduction of PG, glycerol has the additional effect of down-regulatingphosphatidic acid (PA), which, as demonstrated in the Examples below,may also play a role in keratinocyte modulation.

Functional derivatives of glycerol, including but not limited topropylene glycol, have the same or similar effect as glycerol, in bothincreasing production of a PG functional derivative and indown-regulating the production of PA.

Other embodiments of compositions of the present disclosure may includenonfunctional derivatives of glycerol, such as other primary,non-glycerol based alcohols (e.g. 1-butanol and ethanol) thatdown-regulate PLD2 production of both PG and PA, as demonstrated in theexamples below. Such compositions may or may not also include PG,depending on the desired effect. Compositions including a non-glycerolbased alcohol without PG can inhibit/reduce the production of PA and PG,while compositions including a non-glycerol based alcohol and PG caninhibit/reduce PA production and induce PG-mediated modulation ofkeratinocyte behavior.

Pharmaceutical compositions and unit dosage forms typically also includeone or more pharmaceutically acceptable excipients or diluents.Advantages provided by the active composition, such as, but not limitedto, increased solubility and/or enhanced flow, purity, or stability(e.g., hygroscopicity) characteristics can make them better suited forpharmaceutical formulation and/or administration to patients than theprior art. Pharmaceutical unit dosage forms of the active compositionare suitable for topical, transdermal, oral, mucosal (e.g., nasal,sublingual, vaginal, buccal, or rectal), or parenteral (e.g.,intramuscular, subcutaneous, intravenous, intraarterial, or bolusinjection) administration to a patient. Examples of dosage formsinclude, but are not limited to: tablets; caplets; capsules, such ashard gelatin capsules and soft elastic gelatin capsules; cachets;troches; lozenges; dispersions; suppositories; ointments; cataplasms(poultices); pastes; powders; dressings; creams; plasters; solutions;patches; aerosols (e.g., nasal sprays or inhalers); gels; liquid dosageforms suitable for oral or mucosal administration to a patient,including suspensions (e.g., aqueous or non-aqueous liquid suspensions,oil-in-water emulsions, or water-in-oil liquid emulsions), solutions,and elixirs; liquid dosage forms suitable for parenteral administrationto a patient; and sterile solids (e.g., crystalline or amorphous solids)that can be reconstituted to provide liquid dosage forms suitable forparenteral administration to a patient.

The composition, shape, and type of dosage forms of the activecomposition can vary depending on their use. For example, a dosage formused in the acute treatment of a disease or disorder may contain largeramounts of the active ingredient (e.g., the active composition) than adosage form used in the chronic treatment of the same disease ordisorder. Similarly, a parenteral dosage form may contain smalleramounts of the active ingredient than an oral dosage form used to treatthe same disease or disorder. These and other ways in which specificdosage forms encompassed by this disclosure will vary from one anotherwill be readily apparent to those skilled in the art. (e.g., Remington'sPharmaceutical Sciences, 18th ed., Mack Publishing, Easton, Pa. (1990)).

Typical pharmaceutical compositions and dosage forms can include one ormore excipients. Suitable excipients are well known to those skilled inthe art of pharmacy or pharmaceutics. Whether a particular excipient issuitable for incorporation into a pharmaceutical composition or dosageform depends on a variety of factors well known in the art including,but not limited to, the way in which the dosage form will beadministered to a patient. For example, oral dosage forms such astablets or capsules may contain excipients not suited for use inparenteral dosage forms. The suitability of a particular excipient mayalso depend on the specific active ingredients in the dosage form.

The disclosure further encompasses pharmaceutical compositions anddosage forms that include one or more compounds that reduce the rate bywhich an active ingredient will decompose. Such compounds, which arereferred to herein as “stabilizers,” include, but are not limited to,antioxidants such as ascorbic acid, pH buffers, or salt buffers. Inaddition, pharmaceutical compositions or dosage forms of the disclosuremay contain one or more solubility modulators, such as sodium chloride,sodium sulfate, sodium or potassium phosphate or organic acids. Aspecific solubility modulator is tartaric acid.

Like the amounts and types of excipients, the amounts and specific typeof active ingredient in a dosage form may differ depending on factorssuch as, but not limited to, the route by which it is to be administeredto patients, the condition to be treated, the size of the host, etc.However, typical dosage forms of the compounds of the disclosure includePG a pharmaceutically acceptable salt, or a pharmaceutically acceptablepolymorph, solvate, hydrate, dehydrate, co-crystal, anhydrous, oramorphous form thereof, in an amount of from about 0.05 mg to about 50mg, preferably in an amount of from about 0.25 mg to about 10 mg, andmore preferably in an amount of from about 0.5 mg to 5 mg.

In exemplary embodiments, the PG, a functional derivative thereof, apharmaceutically acceptable salt, or a product thereof can be deliveredin the form of liposomes, optionally mixed with one or more of the aboveadditives. Although the compositions of the present disclosure may bedelivered in any form, for treatment of skin disorders, topical dosageforms may be preferable.

Topical, Transdermal and Mucosal Dosage Forms

Topical dosage forms of the disclosure include, but are not limited to,creams, lotions, ointments, gels, shampoos, sprays, aerosols, solutions,emulsions, and other forms known to one of skill in the art. (e.g.,Remington's Pharmaceutical Sciences, 18th ed., Mack Publishing, Easton,Pa. (1990); and Introduction to Pharmaceutical Dosage Forms, 4th ed.,Lea & Febiger, Philadelphia, Pa. (1985)). For non-sprayable topicaldosage forms, viscous to semi-solid or solid forms comprising a carrieror one or more excipients compatible with topical application and havinga dynamic viscosity preferably greater than water are typicallyemployed. Suitable formulations include, without limitation, solutions,suspensions, emulsions, creams, ointments, powders, liniments, salves,and the like, which are, if desired, sterilized or mixed with auxiliaryagents (e.g., preservatives, stabilizers, wetting agents, buffers, orsalts) for influencing various properties, such as, for example, osmoticpressure. Other suitable topical dosage forms include sprayable aerosolpreparations wherein the active ingredient, preferably in combinationwith a solid or liquid inert carrier, is packaged in a mixture with apressurized volatile (e.g., a gaseous propellant, such as freon), or ina squeeze bottle. Moisturizers or humectants can also be added topharmaceutical compositions and dosage forms if desired. Examples ofsuch additional ingredients are well known in the art.(e.g., Remington'sPharmaceutical Sciences, 18^(th) Ed., Mack Publishing, Easton, Pa.(1990)).

Transdermal and mucosal dosage forms of the active composition include,but are not limited to, creams, lotions, ointments, gels, solutions,emulsions, suspensions, suppositories, ophthalmic solutions, patches,sprays, aerosols, or other forms known to one of skill in the art.{e.g., Remington's Pharmaceutical Sciences, 18th Ed., Mack Publishing,Easton, Pa. (1990); and Introduction to Pharmaceutical Dosage Forms, 4thEd., Lea & Febiger, Philadelphia, Pa. (1985)). Dosage forms suitable fortreating mucosal tissues within the oral cavity can be formulated asmouthwashes, as oral gels, or as buccal patches. Additional transdermaldosage forms include “reservoir type” or “matrix type” patches, whichcan be applied to the skin and worn for a specific period of time topermit the penetration of a desired amount of active ingredient.

Suitable excipients (e.g., carriers and diluents) and other materialsthat can be used to provide transdermal and mucosal dosage formsencompassed by this disclosure are well known to those skilled in thepharmaceutical arts, and depend on the particular tissue or organ towhich a given pharmaceutical composition or dosage form will be applied.With that fact in mind, typical excipients include, but are not limitedto water, phosphate-buffered saline, acetone, ethanol, ethylene glycol,propylene glycol, butane-1,3-diol, isopropyl myristate, isopropylpalmitate, mineral oil, and mixtures thereof, to form dosage forms thatare non-toxic and pharmaceutically acceptable.

Depending on the specific tissue to be treated, additional componentsmay be used prior to, in conjunction with, or subsequent to treatmentwith pharmaceutically acceptable salts of the active composition. Forexample, penetration enhancers can be used to assist in delivering theactive ingredients to or across the tissue. Suitable penetrationenhancers include, but are not limited to: acetone; various alcoholssuch as ethanol, oleyl, and tetrahydrofuryl; alkyl sulfoxides such asdimethyl sulfoxide; dimethyl acetamide; dimethyl formamide; polyethyleneglycol; pyrrolidones such as polyvinylpyrrolidone; Kollidon grades(Povidone, Polyvidone); urea; and various water-soluble or insolublesugar esters such as TWEEN 80 (polysorbate 80) and SPAN 60 (sorbitanmonostearate).

The pH of a pharmaceutical composition or dosage form, or of the tissueto which the pharmaceutical composition or dosage form is applied, mayalso be adjusted to improve delivery of the active ingredient(s).Similarly, the polarity of a solvent carrier, its ionic strength, ortonicity can be adjusted to improve delivery. Compounds such asstearates can also be added to pharmaceutical compositions or dosageforms to advantageously alter the hydrophilicity or lipophilicity of theactive ingredient(s) so as to improve delivery. In this regard,stearates can serve as a lipid vehicle for the formulation, as anemulsifying agent or surfactant, and as a delivery-enhancing orpenetration-enhancing agent. Different hydrates, dehydrates,co-crystals, solvates, polymorphs, anhydrous, or amorphous forms of thepharmaceutically acceptable salt of an active composition can be used tofurther adjust the properties of the resulting composition.

EXAMPLES

Now having described the embodiments of the compositions and methods formodulating and/or normalizing keratinocyte function and/orproliferation, methods of modulating phosphatidylglycerol levels inkeratinocytes, and methods and compositions for treating skin conditionsin general, the following examples describe certain embodiments ofcompositions and methods for modulating and/or normalizing keratinocytefunction and/or proliferation, methods of modulatingphosphatidylglycerol levels in keratinocytes, and methods andcompositions for treating skin conditions. While such embodiments aredescribed in connection with Examples 1-3 and the corresponding text andfigures, there is no intent to limit the embodiments of the presentdisclosure to these descriptions. On the contrary, the intent is tocover all alternatives, modifications, and equivalents included withinthe spirit and scope of embodiments of the present disclosure.

Example 1

This example provides evidence that long-term exposure of keratinocytesto elevated extracellular calcium concentration increases PLD activityand that elevated extracellular calcium, but not 1,25-dihydroxyvitaminD3, increases PLD-mediated phosphatidylglycerol production in cellslabeled with [³H] or [¹⁴C]glycerol. This increase inphosphatidylglycerol production upon chronic elevated extracellularcalcium exposure is not entirely the result of an increase in glyceroluptake. In addition, PMA increases PLD activity but does not enhancephosphatidylglycerol formation. Since (1) PLD-1, but not PLD-2,expression and activity is increased by 1,25-dihydroxyvitamin D₃ and (2)PMA activates PLD-1 to a greater extent than PLD-2, this suggests thatradiolabeled PG production upon exposure to glycerol is a measure ofPLD-2 activation in keratinocytes.

Experimental

Materials

Membranes obtained from Sf9 insect cells overexpressing PLD-2 wereprovided by Onyx Pharmaceuticals, California, U.S. [³H] Oleic acid, [³H]palmitoylphosphatidylcholine, [³H] glycerol {three different forms wereused as products were discontinued: [1,2,3-³H]glycerol (specificactivity of 200 mCi/mmol), [1,2,3-³H] glycerol (specific activity of40-80 mCi/mmol) and [2-³H] glycerol (specific activity of 200 mCi/mmol)}and [1,3-¹⁴C]glycerol were obtained from NEN/DuPont (Boston, Mass.,U.S.). Egg-derived phosphatidylglycerol, soy-derivedphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine andstandards of phosphatidylethanol, phosphatidic acid andphosphatidylglycerol were purchased from Avanti Polar Lipids (Alabaster,Ala., U.S.). Phosphatidylinositol 4,5-bisphosphate was obtained fromCalbiochem (San Diego, Calif., U.S.) or Sigma (St. Louis, Mo., U.S.).Calcium-free MEM and antibiotics were purchased from Biologos, Inc.(Maperville, Ill., U.S.). Bovine pituitary extract, epidermal growthfactor and HEPES solution (1 M, pH 7.4) were obtained from Gibco BRL(Grand Island, N.Y., U.S.). ITS+ was supplied by CollaborativeBiomedical Products (Bedford, Mass., U.S.) and dialyzed fetal bovineserum by Atlanta Biologicals (Atlanta, Ga., U.S.). Silica gel 60 TLCplates with concentrating zone were obtained from EM Science (Gibbstown,N.J., U.S.). AU other reagents were obtained from standard suppliers andwere of the highest grade available.

In Vitro Assay of Phosphatidylglycerol Formation

PLD-2 activity was measured in vitro with[³H-palmitoyl]phosphatidylcholine as substrate. Radiolabeledphosphatidylcholine was incorporated into lipid vesicles prepared fromphosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol4,5-bisphosphate as described in R. D. Griner, F. Qin, E. M. Jung, C K.Sue-Ling, K. B. Crawford, R. Mann-Blakeney, R. J. Bollag, and W. B.Bollag, 1,25-Dihydroxyvitamin D₃ induces phospholipase D-1 expression inprimary mouse epidermal keratinocytes, J. Biol. Chem. 274 (1999)4663-4670, incorporated herein by reference. Glycerol and/or ethanol wascombined with the liposomes and the reaction initiated by the additionof PLD-2-overexpressing Sf9 cell membranes, which were provided by OnyxPharmaceuticals, Richmond, Calif., U.S. The reaction was then allowed toproceed at 37° C. for 30 minutes prior to termination by the addition of0.2% SDS containing 5 mM EDTA. Lipids were extracted according to themethod of Bligh and Dyer and radiolabeled phospholipids separated andquantified, as described in W. B. Bollag, “Measurement of phospholipaseD activity, Methods” Mol. Biol. 105 (1998) 151-160, incorporated hereinby reference.

Cell Culture

Primary epidermal keratinocytes were prepared from 1-3-day old neonatalICR mice after trypsin flotation of the skin and mechanical separationof the epidermis from the dermis. The epidermal cells were released byscraping, collected by centrifugation and plated in 6-well dishes in amedium consisting of MEM containing 25 μM calcium, 2% dialyzed fetalbovine serum, 2 mM glutamine, 5 ng/mL EGF, ITS (6.25 μg/mL insulin+6.25μg/mL transferrin+6.25 ng/mL selenious acid+5.35 μg/mL linoleicacid+1.25% bovine serum albumin), 100 U/mL penicillin, 100 μg/mLstreptomycin and 0.25 μg/mL fungizone. After an overnight incubation,the cells were refed with serum-free keratinocyte medium (SFKM), inwhich 2% dialyzed fetal bovine serum was replaced with 90 μg/mL bovinepituitary extract. Cells were refed with fresh medium every 1-3 days.

PLD Activity and [³HI or [¹⁴C1Phosphatidγlglycerol Formation

For the PLD assay cultured primary keratinocytes were labeled for 20-24hours with 2.5 μCi/ml [³H] oleic acid. The cells were then exposed tovehicle or 100 nM PMA in the presence of 0.5% ethanol for 30 minutes. Tomeasure the formation of radiolabeled phosphatidylglycerol, cells weretreated for 24 hours with SFKM containing vehicle, 250 nM1,25-dihydroxyvitamin D₃ or 125 μM calcium and then labeled for anadditional 30 minutes with 1-2.5 μCi/mL [³H] or 0.4-0.5 μCi/mL[¹⁴C]glycerol. For experiments investigating the extracellular calciumdependence of PG formation, cells were incubated for 24 hours in SFKMcontaining various calcium concentrations prior to the addition of 5μCi/mL [³H]glycerol for 30 minutes. In some cases, cells were stimulatedwith 25 μM calcium (control)—or 125 μM calcium-containing SFKM for 24hours prior to the addition of [¹⁴C]glycerol in the presence and absenceof 1% ethanol. To measure phosphatidylglycerol formation in response toPMA, unlabeled cells were stimulated with 100 nM PMA in the presence ofradiolabeled glycerol, as above. Reactions were terminated and theradiolabeled phosphatidylalcohol extracted, separated by thin-layerchromatography and quantified as described by Bollag (1998), referencedabove.

Demonstration of Radiolabel in the Headgroup Position of[¹⁴C]Phosphatidylglycerol

Keratinocytes pretreated for 24 hours with control (25 μM calcium) or125 μM calcium-containing medium were exposed to 0.4-0.5 μCi/mL[¹⁴C]glycerol for an additional 30 minutes. Lipids were extracted intochloroform/methanol as described above. Dried lipid extracts were thensolubilized in phospho lipase buffer (100 mM Tris, pH 7.4, 6 mMMgCl₂+0.1% Triton-X100) by extensive vortexing and a short incubation at37° C. and approximately half of each extract was transferred into aclean tube. Distilled water (untreated) or 1 IU/mL (final concentration)of Streptomyces chromofuscus PLD (Sigma, St. Louis, Mo.) diluted indistilled water (PLD-treated) was then added to each of the lipidextract samples, which were incubated at 37° C. for 60 minutes. Releasedheadgroups were then separated from phospholipids by extraction into theaqueous layer, essentially according to the method of Folch. J. Rolch,M. Lees, G. H. S. Stanley, “A simple method for the isolation andpurification of total lipids from animal tissues”, J. Biol. Chem. 226(1957) 497-509, incorporated herein by reference. Briefly, 75 μLreaction mixtures were diluted with 1.5 mL of chloroform/methanol (2:1volume:volume) followed by the addition of 300 μL of 0.05 M NaCl. Aportion of the upper aqueous layer was then collected and quantified byliquid scintillation spectrometry. PLD-released radioactivity in theaqueous phase was calculated as the amount released in the PLD-treatedsample minus the amount detected in the corresponding untreated sample.In other experiments, PG was first isolated from lipid extracts bythin-layer chromatography as described above and visualized with iodinevapor. PG was extracted from the thin-layer plate usingchloroform/methanol (2:1 volume:volume) and dried under nitrogen. Theisolated PG was then solubilized, incubated with and without bacterialPLD and extracted as above. Following removal of the aqueous aliquot forcounting, the remaining aqueous phase was aspirated, and the organicphase dried under nitrogen. This lipid extract was then separated bythin-layer chromatography and PG and phosphatidic acid in the samplesquantified as above.

[³H]Glycerol Uptake

Confluent primary keratinocytes were incubated for 30, 60, 90, 120, 300or 600 seconds with SFKM containing 20 mM HEPES (for additional pHbuffering), 1 μCi/mL [³H]glycerol and 0.1% DMSO (control) or 100 nM PMA.Reactions were terminated by washing three times with ice-coldphosphate-buffered saline lacking divalent cations. The cells weresubsequently solubilized in 0.3 M NaOH and aliquots of this extractsubjected to liquid scintillation counting. Counts obtained fromduplicate samples at each time point were averaged and graphed, and alinear equation was determined for each condition. Correlationcoefficients obtained were typically 0.99 or greater (mean correlationcoefficient for control was 0.992±0.002 and for PMA, 0.994±0.001).Slopes obtained from multiple experiments were averaged and analyzedstatistically for significant differences between conditions.

The linearity of glycerol uptake determined above allowed measurement ofuptake at a single time point to determine the effects of othertreatments on this process. Thus, confluent keratinocytes werepreincubated for 30 minutes with 0.1% DMSO (control) or 100 nM PMA priorto measuring [³H]glycerol uptake as above but at 5 minutes only.Similarly, near-confluent primary keratinocytes were incubated for 24hours with SFKM containing various calcium concentrations prior tomeasurement of radiolabeled glycerol uptake for 5 minutes.

Statistical Analysis

The significance of differences between mean values was determined usinganalysis of variance (ANOVA), as performed by the program Instat(GraphPad Software, San Diego, Calif.).

Results

PLD-2 Utilizes Glycerol as a Primary Alcohol for theTransphosphatidylation Reaction in Vitro (Characterization of theResponse)

In intact cells, PLD has the unique property of catalyzing not only thehydrolysis of phospholipids to form phosphatidic acid but also, in thepresence of primary alcohols, a transphosphatidylation reaction thatresults in the production of phosphatidylalcohols. Thus, the generationof phosphatidylalcohols has been used as a measure of PLD activity.Typically, primary alcohols such as ethanol or 1-butanol are used sincethis results in the production of novel phosphatidylalchohols that arenot readily metabolized by the cell. Previous studies in intact cellshave suggested that the physiological primary alcohol, glycerol, canalso serve as a substrate for the transphosphatidylation reaction.PLD2-overexpressing Sf9 membranes, were used to investigate whetherglycerol is a substrate for PLD2 in vitro. As shown in FIG. 5 A, PLD2catalyzed the formation of PG from phosphatidylcholine in the presenceof glycerol. This formation was dependent on the concentration ofglycerol in the reaction mix (FIG. 5A), as well as the amount of PLD2added and the time of incubation (data not shown). Furthermore, glycerolcould compete with the primary alcohol ethanol to generate PG in placeof phosphatidylethanol (FIG. 5B). PLD-1 was also observed to generate PGin vitro in the presence of glycerol (data not shown).

The Production of Radiolabeled Phosphatidyl Glycerol, Formed UponAddition of [³Hl or [“¹⁴C]Glycerol to Intact Cells, is Increased UponExposure of Keratinocytes to an Elevated Calcium Concentration andDecreased with 1,25-Dihydroxyvitamm D₃ Treatment

The inventors have shown previously that thekeratinocyte-differentiating agent, 1,25-dihydroxyvitamin D₃ increasesPLD-1 expression and activity after a 24-hour exposure (see Griner, etal. referenced above). The current example investigated the effect of1,25-dihydroxyvitamin D₃ and another agent that triggers keratinocytedifferentiation, elevated extracellular calcium levels, onphosphatidylglycerol formation in cells pretreated for 24 hours prior toaddition of [³H] glycerol. Based on the previous results, it wasanticipated that 1,25-dihydroxyvitamin D₃ would increase the generationof PG, since this agent stimulated PLD-1 activity and expression.Unexpectedly, exposure to 1,25-dihydroxyvitamin D₃ did not increaseradiolabeled PG formation relative to control cells, and in fact, therewas instead an apparent decrease observed (FIG. 6). On the other hand,pretreatment with 125 μM calcium-containing medium induced an increasein the subsequent production of PG (FIGS. 6A and B). This resultsuggested a possible elevated calcium-induced activation of PLD, or thepossibility that other pathways, such as a mechanism in whichglycerol-3-phosphate is added to CDP-diacylglycerol, might be involvedin PG synthesis.

The Effect of Elevated Calcium Concentrations on PG Production, andGlycerol Uptake, is Dose-Dependent

Elevated extracellular calcium levels induce various stages ofkeratinocyte differentiation in a concentration-dependent manner.Calcium concentrations in the range of 100-125 μM stimulate theexpression of keratin-1, a marker of early (spinous) differentiation,whereas higher concentrations induce markers of later differentiation,e.g., transglutaminase activity. Thus, the dose-dependence of the effectof elevated extracellular calcium levels on PG production wasinvestigated herein. PG formation in response to elevated extracellularcalcium concentrations [over the range 25 μM (control) to 1 mM]exhibited a biphasic dose dependence (FIG. 7A). Thus, maximalstimulation of radiolabeled PG formation was observed at 125 μM calcium,with a gradually declining effect at higher calcium concentrations.

The ability of intermediate calcium concentrations to stimulate PGformation maximally could be the result of an increase in glyceroluptake, an enhancement of PLD activity or both. The effect ofpretreatment of keratinocytes with various calcium concentrations onsubsequent radiolabeled glycerol uptake was determined as described.Pre-exposure to 125 μM and 250 μM calcium-containing medium induced anincrease (of 56% and 41%, respectively) in glycerol uptake relative tothe 25 μM calcium control, whereas glycerol uptake in 500μM-calcium-pretreated keratinocytes was approximately equivalent to thecontrol value (FIG. 7B). On the other hand, a concentration of 1 mMinduced a slight but not significant inhibition (in these experiments)of glycerol uptake. The small increase in glycerol uptake observed with125 μM calcium pretreatment is unlikely by itself to account for thelarge increase in radiolabeled PG production, suggesting that PLD wasalso activated by the intermediate calcium concentrations.

The ability of intermediate calcium concentrations to stimulate PGsynthesis suggested that this process was associated with earlydifferentiation events. Therefore, the effect of an intermediate1,25-dihydroxyvitamin D₃ concentration on PG synthesis was examined,which is also known to stimulate expression of the early differentiationmarker keratin-1 (10 nM). In contrast to the results with theintermediate calcium concentrations, a concentration of1,25-dihydroxyvitamin D₃ did not increase PG synthesis, and in fact,both the intermediate and high (250 nM) concentrations of1,25-dihydroxyvitamin D₃ significantly inhibited PG production (FIG. 8).

Increased Radiolabeled Phosphatidylglycerol Formation Upon Treatmentwith an Elevated Calcium Concentration in Intact Cells is Mediated, atLeast in Part, by PLD

As observed in FIG. 6B, elevated extracellular calcium concentrationappeared to induce an increase not only in the synthesis of PG but alsoof phosphatidylcholine and phosphatidic acid. Therefore, it was possiblethat calcium enhanced general phospholipid synthesis, stimulatingglycerol incorporation into the phospholipid backbone rather than theheadgroup, and that therefore increased PG synthesis occurredindependently of PLD activity. Since ethanol and glycerol both act as asubstrate for the transphosphatidylation reaction (FIG. 5B), ethanol wasused to determine whether elevated extracellular calciumconcentration-elicited stimulation of PG formation occurred through theactivation of PLD. Ethanol (1%) was added to keratinocytes pretreatedwith 125 μM calcium minutes before initiation of PG production with[¹⁴C]glycerol. As shown in FIG. 9, ethanol significantly inhibited PGformation stimulated by preexposure to elevated extracellular calciumlevels, without affecting basal (control) PG production. The ability ofethanol to compete with glycerol suggests that some, if not all,elevated calcium-stimulated PG formation is the result of an enhancementof PLD activity.

The involvement of PLD in elevated extracellular calcium-induced PGsynthesis was further demonstrated by the ability of bacterial PLD torelease radiolabel from lipid extracts and isolated PG. In theseexperiments, cells were pretreated with or without 125 μMcalcium-containing medium for 24 hours prior to addition of[¹⁴C]glycerol for 30 minutes. Lipid extracts were then prepared,solubilized in a Triton X100-containing buffer, and incubated with orwithout bacterial PLD for 1 hour. This bacterial PLD has been used toquantify phosphatidylglycerol in amniotic fluid, through its ability torelease the glycerol headgroup. Released headgroups were thenpartitioned into the aqueous phase using the Folch method, as describedabove. Upon incubation with bacterial PLD, [¹⁴C]glycerol-labeled lipidextracts from 125 μM calcium-pretreated cells released approximatelyfour times the amount of radiolabel into the aqueous fraction as thosefrom control-pretreated cells (control: 1.00±0.09; calcium: 4.2±0.4-foldover the control level; p<0.001 with values representing the means±SEMof 6 samples from 3 separate experiments). This result suggests thatmore glycerol was being incorporated into the headgroup position withcalcium exposure, consistent with enhancement of a PLD-mediatedtransphosphatidylation reaction.

Similar experiments using PG isolated from control or elevatedextracellular calcium-pretreated cells are shown in FIG. 10. Again,bacterial PLD released greater than 3-fold more radioactivity from PGisolated from 125 μM calcium-pretreated cells than from control cells(control: 1.00±0.04; calcium: 3.3±0.5-fold over the control level;p<0.01 with values representing the means±SEM of 6 samples from 3separate experiments). Thin-layer chromatographic analysis of thebacterial PLD-treated and -untreated PG samples demonstrated that aportion of the radiolabeled PG was converted to radiolabeled PA,indicating that some of the glycerol was present in the phospholipidbackbone (FIG. 10). However, only approximately 40% of the originalradiolabel found in PG was recovered in PA, indicating thatapproximately 60% of the radiolabel in PG was present in the headgroupposition.

Phorbol Ester Increases PLD Activity but does not Increase RadiolabeledPG Formation

Another agent known to induce both sustained PLD activity in intactcells and keratinocyte differentiation is the phorbol ester, PMA.Therefore, the effect of PMA on PG formation was determined. PMAactually elicited a significant (p<0.01 by unpaired Student's t-test)decrease in PG production (FIG. 11, right), despite the fact that itsimulated a large increase in PLD activity (p<0.02), monitored using theformation of radiolabeled phosphatidylethanol in [³H]oleate-prelabeledas a measure (FIG. 11, left). The ability of PMA to inhibit radiolabeledPG production could be the result of a PMA-mediated decrease in glyceroluptake. Simultaneous incubation of keratinocytes with [³H]glycerol inthe presence and absence of 100 nM PMA elicited no significant effect onglycerol uptake measured over 10 minutes (FIG. 11, and slope values ofPMA 0.998-±0.009-fold over the control value of 1.00, determined asdescribed in Methods and in reference [25]; n=3). However, pretreatmentof keratinocytes for 30 minutes with vehicle or PMA prior to addition ofradiolabeled glycerol for 5 minutes resulted in a PMA-induced decreasein glycerol uptake (FIG. 12), suggesting an effect of phorbol ester onglycerol transport that needs time (greater than 10 minutes) to develop.These results, together with the inability of 1,25-dihydroxyvitamin D₃to increase PG formation, suggest that the production of PG is not auniversal corollary of PLD activation.

Discussion

An interesting and useful finding has been made with respect to PLD: itsability to utilize primary alcohols for the production of novelphosphatidylalcohols in a transphosphatidylation reaction. Thischaracteristic has been exploited by signal transduction researchers tomeasure PLD activity specifically and to inhibit PLD-mediated signalgeneration. However, the current data demonstrates that there is aphysiological alcohol, glycerol, for which PLD retains this ability touse unphysiological alcohols. Indeed, in in vitro experiments PLD2demonstrates the capacity to utilize glycerol as a substrate for thetransphosphatidylation reaction (FIG. 5). The results furtherdemonstrate that by utilizing glycerol for the transphosphatidylationreaction, PLD generates a potential lipid signaling molecule, PG.

One of the corollaries of the mechanism of PLD-2 utilizes glycerol as aprimary physiological alcohol for the transphosphatidylation reaction isthe colocalization of PLD-2 and the glycerol uptake mechanism. Indeed,in previous work, the inventors of the present disclosure found thatPLD2 was collocated with aquaporin-3 in caveolin-rich membranemicrodomains (See Zhang and Bollag (2003) referenced above). Aquaporin-3protein expression has been shown to localize to the basal layer of theepidermis. Consistent with this result, studies demonstrated decreasedaquaporin-3 mRNA and protein expression, upon stimulation of primarykeratinocytes with the differentiating agents, elevated extracellularcalcium concentration and 1,25-dihydroxyvitamin D₃. The reducedexpression also resulted in inhibited function, in that radiolabeledglycerol uptake was decreased by both elevated extracellular calciumconcentration and 1,25-dihydroxyvitamin D₃. However, there was nosignificant difference in the inhibition by these two agents, suggestingthat their disparate effect on radiolabeled PG production is not due todifferences in their ability to inhibit uptake of the radiolabeledglycerol. On the other hand, the ability of 125 μM calcium to trigger amaximal increase in PG production is likely the result of itsstimulation of PLD activity as well as its lack of inhibition ofglycerol uptake (indeed, pretreatment with this concentration of calciumstimulated glycerol uptake). Inhibition of glycerol uptake by highercalcium concentrations probably explains the biphasic PG productionobserved in response to various calcium concentrations (FIG. 7).Interestingly, PMA also inhibited glycerol uptake (FIG. 12), consistentwith the idea that PKC modulates aquaporin-3 function, as has beenobserved for aquaporin-4. High calcium concentrations are also reportedto stimulate PKC activity, and this might represent the mechanism bywhich elevated calcium levels affect glycerol uptake.

The ability of elevated extracellular calcium concentrations tostimulate PG production, whereas the additional keratinocytedifferentiating agents, 1,25-dihydroxyvitamin D₃ and PMA did not,suggests an important difference in the mechanism by which these threeagents trigger the differentiative response. Thus, maximal elevatedextracellular calcium and 1,25-dihydroxyvitamin D₃ concentrations actsynergistically to increase various markers of keratinocytedifferentiation, rather than less than additively as would be expectedif the two agents utilized a completely common pathway. In addition, PMAis known to produce changes in keratinocytes consistent with inductionof late (granular) differentiation and actually inhibits markers ofearly differentiation, in contrast to the effects of elevatedextracellular calcium and 1,25-dihydroxyvitamin D₃ levels. PLD-1 hasbeen proposed to mediate at least in part, 1,25-dihydroxyvitaminD₃-induced keratinocyte late differentiation, based on the findings thatexogenous (bacterial) PLD can induce keratinocyte differentiation and1,25-dihydroxyvitamin D₃ increases PLD-1 expression and activity. On theother hand, 1,25-dihydroxyvitamin D₃ does not enhance PG formation(FIGS. 6 and 8), nor does PMA (FIG. 11). Since 1,25-dihydroxyvitamin D₃does not increase PLD-2 expression and PMA is reported to activate PLD-1to a greater extent than PLD-2, in keratinocytes radiolabeled PGproduction upon exposure to glycerol may be a measure of PLD-2activation. Thus, this assay provides a way to monitor the activity of asingle PLD, PLD-2, in an intact cell system possessing both PLDisoforms.

An interesting aspect of these studies was the observed formation ofphosphatidylcholine and phosphatidic acid upon addition of radiolabeledglycerol. In PG, the glycerol is presumably incorporated, at least inpart, as the headgroup in a transphosphatidylation reaction, since theincorporation can be inhibited by ethanol (FIG. 9). Indeed, in vitroexperiments utilizing bacterial PLD to release phospholipid headgroups,demonstrated that elevated extracellular calcium pretreatment enhancedthe incorporation of glycerol into the headgroup position (FIG. 10). Inphosphatidylcholine and phosphatidic acid, the glycerol is most likelyincorporated into the phospholipid as a glycerol backbone. Phosphatidicacid is formed de novo by the addition of two fatty acids (viafatty-acyl CoAs) to glycerol 3-phosphate, produced by the action ofglycerol kinase on glycerol; the subsequent addition of choline (viaCDP-choline) to dephosphorylated phosphatidic acid (diacylglycerol)produces phosphatidylcholine. Since radiolabeled glycerol was added fora total of 30 minutes only, this result would suggest rapid and activephospholipid synthesis. This idea is consistent with the role ofkeratinocytes in generating the lipids for forming the waterpermeability barrier of skin. It was also shown that radiolabeledglycerol is incorporated into the backbone of PG as well, accountingpartially for the increase in radiolabeled PG formation. Thus, thepresent results confirm that PG synthesis can occur in at least twoways: through a PLD-mediated transphosphatidylation reaction and via themore traditional route of the addition of glycerol-3-phosphate toCDP-diacylglcyerol and subsequent removal of the phosphate group.

Several possibilities exist for the role of PG in keratinocytes. Basedon the localization of glycerol-transporting aquaporin-3 to the basallayer in skin, one might expect this signaling pathway to function in aproliferative capacity or perhaps in early differentiation events. Thisidea is consistent with the observation that radiolabeled PG productionis stimulated maximally by an intermediate calcium concentration (125μM; FIG. 7) known to induce near-maximal expression of keratin-1, amarker of early differentiation. Such an interpretation would also besupported by the data indicating a role for PG in PKC-βII-mediatedmitotic progression. While a previous study has reported no detectableexpression of PKC-β by northern analysis of mouse keratinocytes, otherstudies in both mouse and human have suggested expression of thisisoform in keratinocytes. On the other hand, recent generation andinitial characterization of an aquaporin-3 null mouse mutant indicatesthe importance of this aquaglyceroporin to normal skin physiology. Thesenull mice display a skin phenotype of dry skin and altered water-holdingcapacity. In addition, absorption of the water through epidermisstripped of its water-impermeable outer layer (the stratum corneum) isabnormal in the aquaporin-3-null mice, suggesting a change in someaspect of the epidermal structure that inhibits its hydrating ability.Based on the present results, it is believed that the decreasedformation of PG in aquaporin-3 null mice results in defects inkeratinocyte growth and/or differentiation that result in the abnormalskin physiology observed in these mutants.

Example 2

This example provides additional evidence for a PLD2/AQP3/glyceol/PGmodule in keratinocytes, demonstrating that glycerol entering through anacid-sensitive aquaglyceroporin is utilized by PLD to form PG. Intransient co-transfection studies AQP3 was co-expressed with reporterconstructs in which promoters for markers of keratinocyte proliferativeor differentiative status drive luciferase expression. These studiesindicated that AQP3 co-expression inhibited the promoter activity ofkeratin 5, a marker of basal, proliferative keratinocytes, increased thepromoter activity of keratin 10, a marker of early keratinocytedifferentiation, and enhanced the effect of an elevated extracellularcalcium level on the promoter activity of involucrin, a marker ofintermediate differentiation. Glycerol and 1,2-propylene glycol(glycerol missing one hydroxyl group on the number 3 terminal carbon)inhibited DNA synthesis in a dose-dependent manner both in a low (25 μM)and an intermediate (125 μM) calcium concentration, whereas equivalentconcentrations of the osmotically active agents, xylitol and sorbitol,had little or no effect. Direct provision of PG liposomes also inhibitedDNA synthesis in a dose-dependent fashion in rapidly dividingkeratinocytes, although in growth-inhibited cells PG liposomes dosedependently enhanced [³H]thymidine incorporation into DNA. A trend forstimulation of transglutaminase activity by PG liposomes was alsoobserved. These data support the idea of a signaling module consistingof AQP3, PLD2, glycerol, and PG and involved in promoting growthinhibition and/or early differentiation of proliferating keratinocytes.

Experimental Procedures

Keratinocyte Preparation and Cell Culture

Keratinocytes were prepared from ICR CD-I outbred mice in accordancewith a protocol approved by the Institutional Animal Care and UseCommittee. Briefly, the skins were harvested and incubated overnight in0.25% trypsin at 4° C. The epidermis and dermis were separated and basalkeratinocytes scraped from the underside of the epidermis. The cellswere collected by centrifugation and incubated overnight in anatmosphere of 95% air/5% carbon dioxide at 37° C. in plating medium asdescribed in Dodd M E, Ristich V L, Ray S, Lober R M, Bollag W B (2005)Regulation of protein kinase D during differentiation and proliferationof primary mouse keratinocytes. J Invest Dermatol 125:294-306,incorporated herein by reference. The plating medium was replaced withserum-free keratinocyte medium (SFKM) also as in Dodd, et al., and thecells were refed every 1-2 days with fresh medium until use.

[³HlGlycerol Uptake Assay

Near-confluent keratinocyte cultures were incubated for 24 hours in SFKM(25 μM calcium) or SFKM containing 125 μM calcium (125 μM Ca²⁺-SFKM) andthe glycerol uptake assay performed as previously described in Zheng &Bollag (2003). Briefly, cells were incubated with SFKM containing 20 mMHEPES (for additional pH buffering) and 1 μCi/mL [³H] glycerol for 5minutes, since it has previously been shown that this time point is inthe linear range of [³H]glycerol uptake (Zheng & Bollag (2003).Reactions were terminated by rapidly washing three times with ice-coldphosphate-buffered saline lacking divalent cations (PBS-). Cells werethen solubilized in 0.3 M NaOH and [³H]glycerol uptake quantified byliquid scintillation spectroscopy.

PG Synthesis

After incubation of near-confluent keratinocytes for 24 hours in SFKM(25 μM calcium) or SFKM containing 125 μM calcium (125 μM Ca²⁺-SFKM),0.5-1 μCi/mL [¹⁴C] glycerol was added for 10 minutes and PG synthesis.Briefly, radiolabeled PG was extracted into chloroform/methanol andseparated by thin-layer chromatography on silica gel 60 plates asdescribed in Zheng, X., Ray, S, and Bollag, W. B. (2003) Biochim.Biophys. Acta, 1643, 25-36, incorporated herein by reference.

Co-Transfection Analysis

Co-transfection experiments were performed as described by Dodd, et al.,using 1 ng of the pcDNA3 empty vector or a construct possessing AQP3, 1ng of one of the reporter constructs in which the promoters for keratin5, keratin 10 or involucrin drive expression of luciferase and 0.25 ngof the pRL-SV40 control vector (included in the Promega Dual LuciferaseReporter Assay kit) to normalize for transfection efficiency. Thekeratin 5 and keratin 10 promoter-luciferase constructs were provided byof Dr. Bogi Andersen (University of California, Irvine, Calif.); theinvolucrin promoter-luciferase construct was provided by Dr. DanielBikle (University of California, San Francisco, Calif.). Sub-confluent(approximately 30%) keratinocytes were transfected usingTransIT-Keratinocyte according to the manufacturer's instructions. After24 hours cells were refed with medium containing 25 μM (control) or 1mM-Ca²⁺ for an additional 24 hours. Luciferase activity was thenmeasured using the Dual Luciferase Reporter Assay kit (Promega, Madison,Wis.) as directed by the manufacturer.

Assay of DNA Synthesis

[³H]Thymidine incorporation into DNA was determined as a measure of DNAsynthesis as previously described by Griner, et al., above.Near-confluent keratinocyte cultures were incubated for 24 hours in SFKMcontaining the indicated additions. PG was added in the form ofliposomes prepared by bath sonication of dried PG in SFKM to make astock solution of 2 mg/mL. [³H]Thymidine at a final concentration of 1μCi/mL was then added to the cells for an additional 1-hour incubation.Reactions were terminated by washing with PBS- and macromoleculesprecipitated with ice-cold 5% trichloroacetic acid. Cells weresolubilized in 0.3 M NaOH and the radioactivity incorporated into DNAquantified by liquid scintillation spectroscopy.

Transglutaminase Assay

Keratinocytes were treated with PG liposomes, collected by scraping andcentrifugation in homogenization buffer and lysed by sonication afterone freeze-thaw cycle. Transglutaminase activity was monitored in thebroken cells as the amount of [³H]putrescine cross-linked todimethylated casein as described in Bollag, W. B., Zhong, X., Dodd, M.E., Hardy, D. M., Zheng, X. and Allred, W. T. (2005) J. Pharm. Exp.Ther., 312, 1223-1231, incorporated herein by reference. Thecross-linked putrescine-casein was precipitated with tricholoroaceticacid and collected by filtration. Data were normalized to the quantityof protein in each sample, determined using the Biorad protein assaywith bovine serum albumin as standard, and expressed relative to theappropriate control.

Statistics

Experiments were performed a minimum of three times as indicated. Valueswere analyzed for statistical significance by analysis of variance(ANOVA) with a Student-Newmann-Keuls post-hoc test using Instat(GraphPad Software, San Diego, Calif.).

Results

Inhibition of Glycerol Uptake with Acidic Medium Inhibits PG Synthesis

As discussed above, the present inventors have previously shown thatPLD2 and AQP3 colocalize in caveolin-rich membrane microdomains inkeratinocytes. In addition, PLD-mediated PG synthesis is stimulated byelevated extracellular calcium levels in keratinocytes as shown, and itappears that AQP3 provides glycerol to PLD2 for thetransphosphatidylation reaction to produce PG. Since in lung cells AQP3is inhibited by acidic medium, whether a medium of low pH would inhibitglycerol uptake and PG synthesis was investigated. Keratinocytes wereincubated for 24 hours with control SFKM (25 μM Ca²⁺) or SFKM containing125 μM Ca²⁺ prior to measurement of [³H]glycerol uptake and [¹⁴C]PGproduction in SFKM of pH 4 or 7.4. As shown in FIG. 13A, 125 μM Ca²⁺significantly stimulated glycerol uptake in control medium. Low pHmedium significantly inhibited glycerol uptake both under basalconditions and upon stimulation with the intermediate calciumconcentration (FIG. 13A). Similarly, pH 4 medium significantly inhibitedradiolabeled PG synthesis after a 10-minute incubation with[¹⁴C]glycerol both in cells incubated with control medium and 125 μMCa²⁺ medium (FIG. 13B). In order to ensure that the inhibition ofglycerol uptake and/or PG production by pH 4 medium was not related totoxicity, some cells were also preincubated for 5 minutes with pH 4medium prior to measurement of glycerol uptake or PG synthesis incontrol pH 7.4 medium (pH 4/7). Preincubation with pH 4 medium hadessentially no effect on glycerol uptake or PG production (FIG. 13).

Co-expression of AQP3 Inhibits Keratin 5 Promoter Activity, StimulatesKeratin 10 Promoter Activity and Enhances the Effect of an ElevatedExtracellular Calcium Level on Involucrin Promoter Activity

Primary mouse epidermal keratinocytes can be difficult to transfect withhigh efficiency. To overcome this limitation, the cells wereco-transfected with AQP3 or the empty vector and reporter constructs inwhich promoters for markers of keratinocyte proliferation ordifferentiation control luciferase expression as described by Dodd, etal. Since vectors are mixed thoroughly prior to transfection, cells thattake up one vector can incorporate the other, allowing measurement ofreporter luciferase activity only in cells that also possess AQP3 or theempty vector. Whereas keratin 5 expression characterizes basalproliferating keratinocytes, keratin 10 and involucrin mark thedifferentiating spinous cells, with keratin 10 serving as a marker forearly differentiation and involucrin as a marker for intermediatedifferentiation. FIG. 14A illustrates the effect of AQP3 co-expressionon keratin 5 promoter activity under basal conditions and after a24-hour incubation with the differentiating agent, 1 mM calcium. AQP3co-expression induced a significant decrease (to 49±12% of the emptyvector-transfected control) in keratin 5 promoter activity. Calcium (1mM) also inhibited keratin 5 promoter activity (by 64%) and there was nosignificant additional effect of AQP3 co-expression. On the other hand,AQP3 co-expression stimulated keratin 10 promoter activity (FIG. 14B).Treatment with 1 mM calcium inhibited keratin 10 expression by 22%, andthis effect was partially reversed by AQP3 co-expression. As adifferentiating agent, 1 mM calcium might be expected to increasekeratin 10 promoter activity; however, such high calcium concentrationsdrive keratinocytes towards later differentiation and actually reducethe expression of early differentiation markers. Finally, AQP3co-expression had no significant effect on involucrin promoter activityalone but enhanced the stimulation induced by 1 mM calcium (FIG. 14C).These results are consistent with AQP3 co-expression promoting earlykeratinocyte differentiation.

Glycerol and 1,2-Propylene Glycol, but not Xylitol or Sorbitol, InhibitDNA Synthesis

The AQP3 and PLD2 appear to colocalize to provide glycerol for use byPLD2 in the transphosphatidylation reaction to generate PG, which thenacts to promote early keratinocyte differentiation. This suggests thatincreasing the delivery of glycerol through the AQP3 channel can alsotrigger early differentiation. Since one of the first hallmarks of earlydifferentiation is exit from the cell cycle and a reduction in DNAsynthesis, the effect of exogenous glycerol (to enhance flux through thechannel) on [³H]thymidine incorporation into DNA, a measure of DNAsynthesis, was investigated. As shown in FIG. 15A concentrations ofglycerol as low as 0.02% (=2.73 mM) significantly inhibited keratinocyteDNA synthesis. The effects of higher concentrations of glycerol werealso investigated. However, because osmotic stress regulateskeratinocyte function, to control for any osmotic effects of glycerolequivalent concentrations of two other osmolytes, xylitol and sorbitol,were also used as controls. As shown in FIG. 15B, glycerol atconcentrations from 0.1 to 1% inhibited DNA synthesis and enhanced theinhibitory effect of 125 μM Ca²⁺. On the other hand, xylitol had nosignificant effect on basal or 125 μM Ca²⁺-inhibited DNA synthesis.Similarly, we observed no significant effect of sorbitol on eithercontrol or 125 μM Ca²⁺-reduced [³H]thymidine incorporation into DNA(FIG. 15C).

To determine whether glycerol inhibition of proliferation inkeratinocytes was related to AQP3, the effect of AQP3 transfection, withand without added glycerol, on keratin 5 promoter activity was examined.As shown in FIG. 15D, transfection of keratinocytes with AQP3 inhibitedkeratin 5 promoter activity relative to control (transfection with emptyvector). Glycerol (0.2%) induced a small but significant reduction inkeratin 5 promoter activity (about a 9% reduction) Keratinocytestransfected with AQP3 and treated with glycerol (0.2%) showed anenhanced inhibition of keratin 5 promoter activity (about a 47%reduction). This result suggests that AQP3 expression enhanced theinhibitory effect of glycerol on the basal-like phenotype ofkeratinocytes.

PG was also examined for its ability to stimulate a marker ofkeratinocyte differentiation. Keratinocytes were treated with or withoutPG liposomes in 25 uM Ca²⁺ (control) or 125 uM Ca²⁺-containing mediumfor 24 hours and cell lysates were analyzed for involucrin proteinlevels (normalized to actin as a loading control) (FIG. 15E). PGliposomes lone and 125 uM Ca²⁺ had not significant effect on involucrinprotein levels. However, the combination of PG liposomes and moderatelyelevated Ca²⁺ produced a significant increase in involucrin proteinlevels. This result suggests that the AQP3, PLD2, glycerol and PGsignaling module participates in early keratinocyte differentiation butthat later differentiation requires the provision of additional signals(which may be triggered by the early differentiation pathway).

In studies of the AQP3 null mutant mouse, glycerol, but not xylitol or1,2-propylene glycol (or 1,3-propylene glycol), could correct theepidermal phenotype of this knockout model. Therefore, 1,2-propyleneglycol was also tested for its ability to inhibit DNA synthesisbasically and upon differentiation with 125 μM Ca²⁺. The effect of1,2-propylene glycol was analogous to that of glycerol, exhibiting dosedependent inhibition of [³H]thymidine incorporation under control (25 μMCa²⁺) conditions and upon differentiation with 125 μM Ca²⁺ (FIG. 16A).Also shown in FIG. 16B are the structures of glycerol and 1,2-propyleneglycol to demonstrate their similarity.

PG Liposomes Inhibit DNA Synthesis in Rapidly Dividing Keratinocytes andStimulate Transglutaminase Activity

It is further believed that direct provision of PG itself will alsotrigger early differentiation. Providing PG in the form of liposomesdirectly to keratinocytes was found to inhibit DNA synthesis in highlyproliferative cells (FIG. 17A). Maximal inhibition was observed at 25μg/mL with a plateau from 50 to 100 μg/mL. This effect is not likely torepresent toxicity since morphologic changes characteristic of celldeath were not observed (data not shown). There existed the possibilitythat PG liposomes inhibited DNA synthesis nonspecifically. To test thishypothesis, keratinocytes were treated with liposomes fromdioleoyl-phosphatidylpropanol (PP) and DNA synthesis was determined asdiscussed above. As above, PG liposomes inhibited DNA synthesis; howeverPP liposomes showed no impact on DNA synthesis in highly proliferativekeratinocytes (FIG. 17C). It was also shown that dipalmitoyl-PP did notaffect DNA synthesis in highly proliferative keratinocytes (data notshown).

In addition, PG liposomes induced a dose-dependent trend towardsincreased transglutaminase activity, a marker of late keratinocytedifferentiation (FIG. 17B).

PG Liposomes Stimulate DNA Synthesis in Slowly Proliferating Cells

Additional evidence for a lack of toxicity was provided by the observedeffects of the PG liposomes on keratinocytes exhibiting reducedproliferation presumably as the result of contact inhibition. Thus, ifPG liposomes were applied to keratinocytes with decreased proliferativecapacity (as indicated by reduced [³H]thymidine incorporation into DNAunder control conditions), DNA synthesis was stimulated in adose-dependent manner, with a half-maximal effect at a concentration ofapproximately 35 μg/mL and a maximal stimulation at 100 μg/mL (FIG. 18).This result suggests that PG has the capacity to normalize keratinocyteproliferation, inhibiting the proliferation of rapidly dividing cellsand increasing proliferation in a setting of reduced growth.

Discussion

The ability of PLD to utilize glycerol in a transphosphatidylationreaction to synthesize PG, and the interaction between PLD2, and AQP3,suggested a mechanism by which glycerol could reach this isoenzyme forthe transphosphatidylation. This inhibition of the glycerol uptakefunction of AQP3 can reduce PG synthesis as well. FIG. 13 shows thatacidic medium induces a concomitant decrease in 125 μM Ca²⁺-elicitedglycerol uptake and PG synthesis. However, since other aquaporins arecapable of transporting glycerol, such as aquaporin-9, and are expressedby keratinocytes these other aquaglyceroporins may also contribute toglycerol uptake and PG synthesis in keratinocytes.

It is believed that the PG synthesized by the PLD2/AQP3 signaling moduleserves as a lipid messenger to regulate keratinocyte and epidermalfunction. AQP3 null mutant mice exhibit an epidermal phenotype that canbe corrected by glycerol but not other osmotically active agents. Thepresent co-expression studies suggest that AQP3 promotes earlykeratinocyte differentiation: AQP3 decreased the promoter activity ofkeratin 5 (FIG. 14A), a marker of the basal proliferative layer.Downregulation of keratin 5 expression characterizes the transition ofbasal keratinocytes into the first suprabasal cells in the spinouslayer. Also characteristic of spinous keratinocytes is an increase inthe expression of keratin 10; co-expression of AQP3 increased keratin 10promoter activity (FIG. 14B). High calcium levels may propelkeratinocytes past early differentiation steps to a laterdifferentiation stage, resulting in a slight reduction in keratin 10promoter activity (FIG. 14B). As keratinocytes proceed to migrate upthrough the multiple spinous layers, they begin to express involucrin.Although AQP3 co-expression alone did not significantly increaseinvolucrin promoter activity, AQP3 did enhance the effect of anotherdifferentiating agent, elevated extracellular calcium concentration onthe promoter activity of this intermediate differentiation marker (FIG.14C). It should be noted that it seems unlikely that AQP3 is directlyaffecting the promoter activities of these various markers, i.e. viainteractions with other transcription factors and/or the promotersthemselves. Rather, the results are consistent with AQP3 expressioninducing an early differentiation phenotype, and that thedifferentiation status of the cells then controls the activities ofthese promoters.

It is believed that increasing glycerol influx will promote PG synthesisand promote this early differentiation phenotype, a primary event ofwhich is growth arrest. Indeed, glycerol inhibited DNA synthesis andthis inhibition was not reproduced by equivalent concentrations of twoother osmotically active compounds, xylitol and sorbitol (FIG. 15),suggesting that the inhibition was not the result of increasedosmolality. Interestingly, 1,2-propylene glycol (1,2-propanediol)produced an essentially identical effect as glycerol on DNA synthesis(FIG. 16). It is believed that the phospholipid formed bytransphosphatidylation with 1,2-propylene glycol (PG missing the hydroxygroup on the terminal carbon) is similar enough to PG to activate PGeffector enzymes.

If glycerol functions to alter keratinocyte proliferation by serving asa substrate for PG formation, then direct provision of PG would alsoinhibit DNA synthesis. Indeed, in rapidly growing cells (as determinedby high [³H]thymidine incorporation into DNA under basal conditions), PGdose-dependently decreased DNA synthesis (FIG. 17). This effect did notseem to be the result of non-specific toxicity as no morphologicalcorrelates of toxicity were observed (data not shown). In addition,increasing PG doses also showed a tendency to stimulate transglutaminaseactivity, a marker of late keratinocyte differentiation. However,unexpectedly, in keratinocytes that exhibited reduced DNA synthesis,likely as the result of contact inhibition, PG dose dependentlystimulated DNA synthesis (FIG. 18). The mechanism of this biphasicresponse is unknown (although possibilities are discussed below), but incases where the epidermis is hyperproliferative, PG liposomes would beexpected to inhibit keratinocyte growth, whereas under conditions of toolittle proliferation (e.g., with age) the liposomes should increasegrowth. Thus, the results suggest that PG liposomes might be an idealtreatment to normalize skin function under both pathological andphysiological conditions.

The effector enzyme for the PG signal is also unknown; however,possibilities include PG-sensitive protein kinases such as proteinkinase C-II, PKC-, and Pk-P. Alternatively, PG may be incorporated intothe plasma membrane and/or specific microdomains and influence membraneprotein assembly and/or microdomain function. As an example, PG isutilized in photosystem assembly in thylakoid membranes of cyanobacteriaand spinach. PG is also a precursor of cardiolipin(=diphosphatidylglycerol), and both PG and cardiolipin are important inmitochondrial function. Cardiolipin binds to cytochrome c, and oxidationof this lipid is thought to allow release of cytochrome c from themitochondria, an event that can initiate apoptosis. In addition, theincubation of both cardiolipin and PG with depleted mitochondria canpartially restore their membrane potential and this opposes cytochrome crelease and apoptosis. Indeed, PG can inhibit apoptosis in retinalepithelial cells. Thus, PG may induce growth inhibition of rapidlyproliferating keratinocytes (as in FIG. 17A) through activation of aprotein kinase pathway, whereas this phospholipid may promoteproliferation in inhibited cells (as in FIG. 18) by improvingmitochondrial function and energy production. The observed upregulationof AQP3 expression by exposure to ultraviolet light is believed to be acellular response to promote PG production, mitochondrial health andrecovery from the stress of the irradiation. Thus, the novel signalingmodule consisting of AQP3, PLD2, glycerol and PG represents a mechanismfor the beneficial effects of glycerol in skin. Further, the presentresults indicate that this module is an important modulator ofkeratinocyte growth and differentiation in vitro and in vivo andprovides novel treatments for various skin disorders and/or conditions.

Example 3 Glycerol and Phosphatidylglycerol Accelerate Wound Healing

This example presents recent data on the effects of glycerol andphosphatidylglycerol treatment on wound healing obtained in ICR CD1mice. Two full-thickness skin punch biopsies of˜4 mm were made on thebacks of a total of sixteen mice. For each mouse, one wound was either(a) untreated, (b) treated with 2M glycerol in water, (c) treated withphosphate-buffered saline lacking divalent cations (PBS-), or (d)PBS-containing 100 μg/mL phosphatidylglycerol (sonicated to formliposomes). The rate of wound healing was then followed over four daysby digital photography and computer image analysis. Shown in FIG. 19, asa bar graph, is the percentage of wound healing on day 4, relative today 1, for each of the four groups. Glycerol treatment improved the rateof wound healing, as anticipated. More importantly, PG liposomes alsoincreased the rate of wound healing, and this improvement wasstatistically significant. These results validate the idea of theimportance of PG in skin function.

Example 4

This example presents evidence that various PG species and functionalderivatives of PG are capable of modulating keratinocyte differentiationand keratinocyte and epidermal function.

The data presented herein used egg-derived PG that predominatelyincluded palmitic acid and oleic acid as the major fatty acid moleculesin the R₁ and R₂ positions (derived from the phosphatidylcholinemoiety). However, as discussed herein, various species of PG may be usedthat contain a variety of fatty acid molecules in the R₁ and R₂positions.

The fatty acid molecules in the R₁ and R₂ positions may be saturated,monounsaturated or polyunsaturated. The nature of the fatty acidmolecules at the R₁ and R₂ positions may be the same or may bedifferent; for example, one fatty acid molecule may be myristic acid (a14 carbon saturated fatty acid molecule) and one fatty acid may bevaccenic acid (a 18 carbon monunsaturated fatty acid molecule). In oneembodiment, the PG molecule contains fatty acids without unsaturatedbonds, such as, but not limited to, myristic or palmitic. In analternate embodiment, the PG molecule contains at least one fatty acidwith one unsaturated bond, such as but not limited to, oleic orpalmitoleic. In another embodiment, the PG molecule contains at leastone fatty acid with at least two unsaturated bonds, such as but notlimited to, linoleic or arachidonic. The fatty acid molecules in the R₁and R₂ positions may contain from 4-28 carbon atoms and from 0-6unsaturated bonds. Exemplary fatty acid molecules, include, but are notlimited to, butyric (4:0), valeric (5:0), caproic (6:0), caprylic (8:0),capric (10:0), lauric (12:0), myristic (14:0), myristoleic (14:1,cis-9), palmitic (16:0), palmitoleic (16-1,9-cis), stearic (18:0), oleic(18:1, 11-cis), vaccenic (18:1, 11-trans), linoleic (18:2, 9-cis12-cis), -γ-linolenic (18:3, 6-cis 9-cis 12-cis), α-linolenic (18:3,9-cis 12-cis 15-cis), arachidic (20:0), arachidonic (20:4, 5-cis, 8-cis,11-cis 14-cis), eicosapentaenoic (20:5, 5-cis 8-cis 11-cis 14-cis17-cis), behenic (22:0), erucic (22:1, 13-cis), docosahexaenoic (22:6,4-cis 7-cis 10-cis 13-cis 16-cis 19-cis), lignoceric (24:0) and cerotic(26:0).

Furthermore, various headgroups may be incorporated to producefunctional derivatives of PG through the use of primary alcoholsubstrates other than glycerol in the transphosphatidylation reaction toproduce the corresponding phosphatidylalcohol.

This example demonstrates that the nature of the fatty acid moleculeincorporated into the PG or functional derivative thereof and the natureof the headgroup incorporated into functional derivatives of PG canimpact the activity of the PG or functional derivative thereof inmodulating keratinocyte function.

Experimental Procedures

Keratinocyte Preparation and Cell Culture

Keratinocytes were prepared from ICR CD-1 outbred mice as described in

Example 2

Assay of DNA Synthesis

[³H]Thymidine incorporation was used as a measure of DNA synthesis andwas performed as described in Example 2. Liposomes containing PG andfunctional derivatives thereof were prepared by bath sonication asdescribed in Example 2, with the exception that different concentrationsof material were employed.

Statistics

Experiments were performed a minimum of three times as indicated. Valueswere analyzed for statistical significance by analysis of variance(ANOVA) with a Student-Newmann-Keuls post-hoc test using Instat(GraphPad Software, San Diego, Calif.).

Results

Dilinoleoyl-PG (DLPG), Palmitoyl-Arachidonoyl-PG (PAPG) andPalmitoyl-Linoleoyl-PG (PLPG) Inhibit DNA Synthesis in Rapidly DividingKeratinocytes

Various PG species containing selected fatty acid molecules at the R₁and R₂ positions were tested for their ability to inhibit DNA synthesisin rapidly dividing keratinocytes. Providing DLPG, PAPG and PLPG in theform of liposomes directly to keratinocytes was found to inhibit DNAsynthesis in a dose dependent manner in highly proliferative cells (FIG.20A). DLPG, PAPG and PLPG inhibited DNA synthesis at least as stronglyas egg-derived PG. DLPG (18:2-18:2), PAPG (16:0-20:4) and PLPG(16:0-18:2) each contain at least one fatty acid with at least twounsaturated bonds (i.e., a polyunsaturated fatty acid), For DLPG,maximal inhibition was observed at 100 μg/mL with inhibition observed atconcentrations as low as 6.25 μg/mL. For PAPG, maximal inhibition wasobserved at 100 μg/mL with inhibition observed at concentrations as lowas 12.5 μg/mL. For PLPG maximal inhibition was observed at 100 μg/mLwith inhibition observed at concentrations as low as 50 μg/mL.

In addition to DLPG, PAPG and PLPG, palmitoyl-oleoyl-PG (POPG,16:0-18:1) showed moderate inhibition of DNA synthesis (FIG. 20A). POPGexhibited inhibition only at the highest concentration tested (100μg/mL).

As discussed above, these effects are not likely to represent toxicitysince morphologic changes characteristic of cell death were not observed(data not shown).

Furthermore, soy-derived PG was a more potent inhibitor of DNA synthesisthan egg-derived PG. On average, soy-derived PG (from Avanti PolarLipids) contains 59% 18:2, 13% 18:1 and 17% 16:0 fatty acids (plus otherspecies at less than 10%) while egg-derived PG (from Avanti PolarLipids) contains 34% 16:0, 32% 18:1, 18% 18:2 and 11% 18:0 fatty acids(plus other species at less than 10%). POPG, which was a weakerinhibitor, contains one fatty acid with one unsaturated bond and onefatty acid with no unsaturated bonds. DOPG also followed this trend andshowed no inhibition of DNA synthesis.

These data show that the fatty acid composition of the PG species canimpact its ability to modulate keratinocyte differentiation (for examplein this case by stimulating differentiation) and that the degree ofunsaturation in the fatty acid composition of the PG species can impactthe ability of PG species to inhibit DNA synthesis in rapidlyproliferating keratinocytes.

Dioleoyl-PG (DOPG) and Dipalmitoyl-PG (DPPG) Stimulate DNA Synthesis inSlowly Dividing Keratinocytes

As discussed herein, PG is able to inhibit DNA synthesis in rapidlyproliferating cells but stimulate DNA synthesis in slowly proliferatingcells. Various PG species containing selected fatty acid molecules atthe R₁ and R₂ positions were tested for their ability to stimulate DNAsynthesis in slowly dividing keratinocytes. DOPG was shown to be apotent stimulator of DNA synthesis in slowly dividing keratinocytes(FIG. 20B). Stimulation was maximal at 100 μg/mL and could be observedas low as 12.5 μg/mL. DOPG stimulated DNA synthesis more potently thanegg-derived PG.

DPPG stimulated DNA synthesis at approximately the same level asegg-derived PG, but the stimulatory effects were only apparent atconcentrations of 50 μg/mL and higher. Dihexanoyl PG (DHPG, 6:0-6:0),POPG and PAPG were only mildly stimulatory. The stimulatory effect ofcertain PG liposomes also provides additional evidence for a lack oftoxicity of the observed effects of the PG liposomes on keratinocytesexhibiting reduced proliferation presumably as the result of contactinhibition.

These data show that the fatty acid composition of the PG species canimpact its ability to modulate keratinocyte differentiation (for examplein this case by inhibiting differentiation). These results also showthat PG species have the capacity to normalize keratinocyteproliferation, inhibiting the proliferation of rapidly dividing cellsand increasing proliferation in a setting of slowly dividing cells, andthat the nature of the fatty acid molecules incorporated into the PGspecies can impact this normalization of keratinocyte proliferation.

The Identity of the Headgroup Impact the Ability of PG and FunctionalDerivatives Thereof to Modulate Keratinocyte Differentiation

As discussed above, the identity of the headgroups may impact theability of PG and functional derivatives of PG to modulate keratinocytedifferentiation. Various headgroups may be incorporated through the useof primary alcohol substrates other than glycerol in thetransphosphatidylation reaction to produce the correspondingphosphatidylalcohol. Selected phosphatidylalcohol molecules wereproduced using glycerol (to produce PG) or 1-propanol (to producephosphatidylpropanol, PP) as the primary alcohol in thetransphosphatidylation reaction. Various fatty acid molecules were alsotested in each of the PG or PP derivatives produced to generatedipalmitoyl-PG (DPPG, 16:0-16:0), dioleoyl-PG (DOPG, 18:1-18:1),dilinoleoyl-PG (DLPG, 18:2-18:2), dipalmitoyl-PP (DPPP, 16:0-16:0),dioleoyl-PP (DOPP, 18:1-18:1) and dilinoleoyl-PP (DLPP, 18:2-18:2). Thestructure of glycerol and 1-propanol is shown in FIG. 21A.

The above-identified species were tested for their ability to inhibitDNA synthesis in rapidly dividing keratinocytes (FIG. 21B). The resultsare expressed as percent of control (with control being no addition ofany PG or PP species). As can be seen the identity of the headgroup andthe nature of the fatty acid molecule impacted the ability of thespecies to inhibit DNA proliferation in rapidly dividing keratinocytes.In general, PG species were more effective in inhibiting DNA synthesisthan PP species (compare DLPG to DLPP in FIG. 21B). DLPG inhibited DNAsynthesis at the lowest concentration tested (25 μg/mL) while DLPPinhibited DNA synthesis only at the highest concentration tested (100μg/mL). It should be noted that inhibition of DNA synthesis by DLPP wasnot statistically significant as compared to control.

Also, as shown in FIG. 20A, PG species containing dilinoleoyl as thefatty acid constituent were more effective in inhibiting DNA synthesisthan PG species containing dipalmitoyl or dioleoyl as the fatty acidconstituents (compare DLPG to DPPG and DOPG in FIG. 21B). The same wastrue of PP species (FIG. 21B); the data in FIG. 21B is consistent withthe lack of effect of DPPP and DOPP shown in FIG. 17C.

These data show that the identity of the headgroup and the fatty acidcomposition can impact the ability of PG and functional derivatives ofPG to modulate keratinocyte differentiation and can impact the abilityof such species to inhibit DNA synthesis in rapidly proliferatingkeratinocytes. These results also show that PG as a headgroup is moreeffective in normalizing keratinocyte function (in this case inhibitingDNA synthesis in rapidly proliferating cells). Likewise, the data inFIG. 21 also confirm the nature of the fatty acid in the relevantspecies impacts the ability of PG and functional derivatives of PG tomodulate keratinocyte differentiation. Consistent with the data in FIG.20A, only those species incorporating fatty acid molecules with morethan one unsaturated bond were effective in inhibiting DNA synthesis.

It should be emphasized that the above-described embodiments of thepresent disclosure are merely possible examples of implementations, andare set forth only for a clear understanding of the principles of thedisclosure. Many variations and modifications may be made to theabove-described embodiments of the disclosure without departingsubstantially from the spirit and principles of the disclosure. AU suchmodifications and variations are intended to be included herein withinthe scope of this disclosure and protected by the following claims.

What is claimed:
 1. A composition for treating a skin condition, thecomposition consisting essentially of an amount of adilinoleoyl-phosphatidylglycerol optionally in a pharmaceuticallyacceptable carrier or excipient effective to treat the skin condition.2. The composition of claim 1, wherein the skin condition ischaracterized by skin cell hyper-proliferation.
 3. The composition ofclaim 1, wherein the skin condition is selected from at least one of thefollowing: psoriasis, eczema, actinic keratosis, atopic dermatitis,basal cell carcinoma, non-melanoma skin cancer, and unregulated celldivision.
 4. A composition for treating a skin condition, thecomposition consisting essentially of a phosphatidylglycerol andglycerol, optionally in a pharmaceutically acceptable carrier orexcipient, in an amount effective to treat the skin condition.
 5. Thecomposition of claim 1, wherein the dilinoleoyl-phosphatidylglycerolinhibits nucleic acid synthesis.
 6. A composition for treating a skincondition, the composition comprising a lipid component, wherein thelipid component consists essentially of one or more ofdilinoleoyl-phosphatidylglycerol,palmitoyl-arachidonyl-phosphatidyalglycerol, andpalmitoyl-linoleoyl-phosphatidylglycerol, and wherein the lipidcomponent is in an effective amount to treat the skin condition.
 7. Thecomposition of claim 6, further comprising glycerol.
 8. The compositionof claim 1, wherein the lipid component is in an amount effective toinhibit DNA synthesis by more than 50% when applied to dividingkeratinocytes relative to a control.
 9. The composition of claim 4,wherein the lipid component is in an amount effective to inhibit DNAsynthesis by more than 50% when applied to dividing keratinocytesrelative to a control.
 10. The composition of claim 6, wherein the lipidcomponent is in an amount effective to inhibit DNA synthesis whenapplied to skin cells by more than 50% relative to a control.
 11. Aliposome composition for treating a skin condition, the liposomecomposition comprising an effective amount of liposomes consistingessentially of a lipid selected from the group consisting ofdilinoleoyl-phosphatidylglycerol,palmitoyl-arachidonyl-phosphatidyalglycerol, andpalmitoyl-linoleoyl-phosphatidylglycerol, to inhibit DNA synthesis ofdividing keratinocytes relative to a control.
 12. The liposomecomposition of claim 11, further comprising glycerol.